Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family.

Literature DB >> 9118230

TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family.

J Nakayama¹, M Saito, H Nakamura, A Matsuura, F Ishikawa.

Abstract

We have cloned and characterized the rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80. The cDNA encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230. The anti-TLP1 antibody specifically immunoprecipitated the telomerase activity. Moreover, p240 and p230 were copurified with telomerase activity in a series of extensive purification experiments. These results strongly suggest that the TLP1 proteins are components of, or are closely associated with, the rat telomerase. A pulse-chase experiment showed that p240 is modified to p230 in vivo. p230 was the dominant form in telomerase-positive cells, suggesting that modification of the TLP1 protein may regulate telomerase activity in vivo.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1997 PMID： 9118230 DOI： 10.1016/s0092-8674(00)81933-9

Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582

Keyword Cloud
Cited

95 in total

1. Two inactive fragments of the integral RNA cooperate to assemble active telomerase with the human protein catalytic subunit (hTERT) in vitro.

Authors: V M Tesmer; L P Ford; S E Holt; B C Frank; X Yi; D L Aisner; M Ouellette; J W Shay; W E Wright
Journal: Mol Cell Biol Date: 1999-09 Impact factor: 4.272

9. Characterization of the interaction between the nuclease and reverse transcriptase activity of the yeast telomerase complex.

Authors: H Niu; J Xia; N F Lue
Journal: Mol Cell Biol Date: 2000-09 Impact factor: 4.272

10. The telomerase RNA pseudoknot is critical for the stable assembly of a catalytically active ribonucleoprotein.

Authors: D Gilley; E H Blackburn
Journal: Proc Natl Acad Sci U S A Date: 1999-06-08 Impact factor: 11.205

TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family.

1. Two inactive fragments of the integral RNA cooperate to assemble active telomerase with the human protein catalytic subunit (hTERT) in vitro.

2. Establishment of novel human esophageal cancer cell line in relation to telomere dynamics and telomerase activity.

3. Identification of functionally important domains in the N-terminal region of telomerase reverse transcriptase.

4. RNA location and modeling of a WD40 repeat domain within the vault.

5. The hTERTalpha splice variant is a dominant negative inhibitor of telomerase activity.

6. Functional requirement of p23 and Hsp90 in telomerase complexes.

7. Polymerization defects within human telomerase are distinct from telomerase RNA and TEP1 binding.

8. Analysis of the structure of human telomerase RNA in vivo.

9. Characterization of the interaction between the nuclease and reverse transcriptase activity of the yeast telomerase complex.

10. The telomerase RNA pseudoknot is critical for the stable assembly of a catalytically active ribonucleoprotein.