Literature DB >> 9115376

Enhanced RACE method using specific enrichment by biotinylated oligonucleotides bound to streptavidin coated magnetic particles.

S Lankiewicz1, G Gisselmann, H Hatt.   

Abstract

RACE (rapid amplification of cDNA ends) is commonly used for identification and isolation of 3'and 5'termini of cDNA. We developed an improvement of the RACE-method that allows the enrichment of wanted fragments. The important new feature is the purification of the amplified products by biotinylated oligonucleotides that hybridize internally. Hybrids are isolated by streptavidin coated magnetic particles.

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Year:  1997        PMID： 9115376      PMCID： PMC146681          DOI： 10.1093/nar/25.10.2037

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  5 in total

1.  Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

Authors:  M A Frohman; M K Dush; G R Martin
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

Review 2.  Cloned glutamate receptors.

Authors:  M Hollmann; S Heinemann
Journal:  Annu Rev Neurosci       Date:  1994       Impact factor: 12.449

3.  Enrichment of microsatellites from the citrus genome using biotinylated oligonucleotide sequences bound to streptavidin-coated magnetic particles.

Authors:  J M Kijas; J C Fowler; C A Garbett; M R Thomas
Journal:  Biotechniques       Date:  1994-04       Impact factor: 1.993

4.  Identification and functional expression of a novel ligand binding subunit of the inhibitory glycine receptor.

Authors:  J Kuhse; V Schmieden; H Betz
Journal:  J Biol Chem       Date:  1990-12-25       Impact factor: 5.157

5.  Molecular cloning of human 5-hydroxytryptamine3 receptor: heterogeneity in distribution and function among species.

Authors:  A Miyake; S Mochizuki; Y Takemoto; S Akuzawa
Journal:  Mol Pharmacol       Date:  1995-09       Impact factor: 4.436
  5 in total
  2 in total

1.  Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms.

Authors:  A Fagotti; G Gabbiani; R Pascolini; P Neuville
Journal:  Nucleic Acids Res       Date:  1998-04-15       Impact factor: 16.971

2.  Sequence and expression characteristics of a nuclear-encoded chloroplast sigma factor from mustard (Sinapis alba).

Authors:  M Kestermann; S Neukirchen; K Kloppstech; G Link
Journal:  Nucleic Acids Res       Date:  1998-06-01       Impact factor: 16.971
  2 in total

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