Literature DB >> 9109405

Cloning and expression of superoxide dismutase from Aquifex pyrophilus, a hyperthermophilic bacterium.

J H Lim1, Y G Yu, I G Choi, J R Ryu, B Y Ahn, S H Kim, Y S Han.   

Abstract

A superoxide dismutase (SOD) gene of Aquifex pyrophilus, a marine hyperthermophilic bacterium, was cloned, sequenced, expressed in Escherichia coli, and its gene product characterized. This is the first SOD from a hyperthermophilic bacterium that has been cloned. It is an iron-containing homo-oligomeric protein with a monomeric molecular mass of 24.2 kDa. The DNA-derived amino acid sequence is more similar to those of known Mn- and Fe-SODs from thermophilic archaea than of Cu, Zn-SODs. The metal binding residues found in all SOD sequences from different species are also conserved in A. pyrophilus SOD. The protein is biochemically active only as an oligomer and is resistant to thermal denaturation.

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Year:  1997        PMID： 9109405     DOI： 10.1016/s0014-5793(97)00262-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  11 in total

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Journal:  J Ind Microbiol Biotechnol       Date:  2007-11-07       Impact factor: 3.346

2.  Stress fermentation strategies for the production of hyperthermostable superoxide dismutase from Thermus thermophilus HB27: effects of ions.

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Journal:  Extremophiles       Date:  2013-09-13       Impact factor: 2.395

3.  Recombinant superoxide dismutase from a hyperthermophilic archaeon, Pyrobaculum aerophilium.

Authors:  M M Whittaker; J W Whittaker
Journal:  J Biol Inorg Chem       Date:  2000-06       Impact factor: 3.358

4.  Adaptive responses to oxygen stress in obligatory anaerobes Clostridium acetobutylicum and Clostridium aminovalericum.

Authors:  Shinji Kawasaki; Yusuke Watamura; Masaki Ono; Toshihiro Watanabe; Kouji Takeda; Youichi Niimura
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

5.  Paleoproterozoic snowball earth: extreme climatic and geochemical global change and its biological consequences.

Authors:  J L Kirschvink; E J Gaidos; L E Bertani; N J Beukes; J Gutzmer; L N Maepa; R E Steinberger
Journal:  Proc Natl Acad Sci U S A       Date:  2000-02-15       Impact factor: 11.205

6.  Characterization of an atypical superoxide dismutase from Sinorhizobium meliloti.

Authors:  R Santos; S Bocquet; A Puppo; D Touati
Journal:  J Bacteriol       Date:  1999-08       Impact factor: 3.490

7.  A cambialistic superoxide dismutase in the thermophilic photosynthetic bacterium Chloroflexus aurantiacus.

Authors:  Vanessa L Lancaster; Russell LoBrutto; Fabiyola M Selvaraj; Robert E Blankenship
Journal:  J Bacteriol       Date:  2004-06       Impact factor: 3.490

8.  Cloning and characterization of a new manganese superoxide dismutase from deep-sea thermophile Geobacillus sp. EPT3.

Authors:  Yanbing Zhu; Guohong Wang; Hui Ni; Anfeng Xiao; Huinong Cai
Journal:  World J Microbiol Biotechnol       Date:  2013-11-17       Impact factor: 3.312

9.  Formation of high-order oligomers by a hyperthemostable Fe-superoxide dismutase (tcSOD).

Authors:  Sha Wang; Zhi-Yang Dong; Yong-Bin Yan
Journal:  PLoS One       Date:  2014-10-14       Impact factor: 3.240

10.  Contributions of the C-terminal helix to the structural stability of a hyperthermophilic Fe-superoxide dismutase (TcSOD).

Authors:  Sha Wang; Yong-Bin Yan; Zhi-Yang Dong
Journal:  Int J Mol Sci       Date:  2009-12-23       Impact factor: 6.208
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