| Literature DB >> 9108303 |
E A Permyakov1, D B Veprintsev, G Y Deikus, S E Permyakov, L P Kalinichenko, V M Grishchenko, C L Brooks.
Abstract
A pH-induced conformational transition was found in bovine prolactin within the physiologically significant pH region from 6.5 to 8.5. The thermal stability of prolactin at pH 6.5 is essentially higher than at pH 8.5. Bovine prolactin binds zinc ions with an apparent association constant of 2 x 10(5) M(-1) at pH 6.5 and 1 x 10(4) M(-1) at pH 8.5. The pH dependence of both thermal stability and zinc binding surrounding the pKa of histidine suggests that these residues plays a key role in the structural integrity of bovine prolactin.Entities:
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Year: 1997 PMID： 9108303 DOI： 10.1016/s0014-5793(97)00203-2
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124