Literature DB >> 9106958

Nested polymerase chain reaction for detection of Ehrlichia risticii genomic DNA in infected horses.

J E Barlough1, Y Rikihisa, J E Madigan.   

Abstract

A nested polymerase chain reaction was developed for amplifying a 529-bp segment of the 16S ribosomal RNA gene of Ehrlichia risticii from equine buffy coat cells. Confirmation of identity of the amplified bands was accomplished by Southern hybridization and DNA sequencing. The study indicated a detection limit of > 10 copies of the target gene, and specificity for E. risticii as based on a panel of test rickettsiae. Ticks (Ixodes pacificus) collected in an area of northern California enzootic for equine monocytic ehrlichiosis were found to be negative for E. risticii DNA.

Entities:  

Mesh：

Substances：

Year:  1997        PMID： 9106958     DOI： 10.1016/s0304-4017(96)01083-7

Source DB:  PubMed          Journal:  Vet Parasitol        ISSN： 0304-4017            Impact factor:   2.738


  9 in total

1.  Comparison of PCR and culture to the indirect fluorescent-antibody test for diagnosis of Potomac horse fever.

Authors:  J Mott; Y Rikihisa; Y Zhang; S M Reed; C Y Yu
Journal:  J Clin Microbiol       Date:  1997-09       Impact factor: 5.948

2.  Laboratory maintenance of the bacterial endosymbiont, Neorickettsia sp., through the life cycle of a digenean, Plagiorchis elegans.

Authors:  Stephen E Greiman; Maksym Tkach; Jefferson A Vaughan; Vasyl V Tkach
Journal:  Exp Parasitol       Date:  2015-07-06       Impact factor: 2.011

3.  Helminthic transmission and isolation of Ehrlichia risticii, the causative agent of Potomac horse fever, by using trematode stages from freshwater stream snails.

Authors:  N Pusterla; J E Madigan; J S Chae; E DeRock; E Johnson; J B Pusterla
Journal:  J Clin Microbiol       Date:  2000-03       Impact factor: 5.948

4.  Quantitative real-time PCR for detection of members of the Ehrlichia phagocytophila genogroup in host animals and Ixodes ricinus ticks.

Authors:  N Pusterla; J B Huder; C M Leutenegger; U Braun; J E Madigan; H Lutz
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

5.  Production and characterization of Ehrlichia risticii, the agent of Potomac horse fever, from snails (Pleuroceridae: Juga spp.) in aquarium culture and genetic comparison to equine strains.

Authors:  G H Reubel; J E Barlough; J E Madigan
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

6.  Detection of Ehrlichia risticii, the agent of Potomac horse fever, in freshwater stream snails (Pleuroceridae: Juga spp.) from northern California.

Authors:  J E Barlough; G H Reubel; J E Madigan; L K Vredevoe; P E Miller; Y Rikihisa
Journal:  Appl Environ Microbiol       Date:  1998-08       Impact factor: 4.792

7.  Transmission rates of the bacterial endosymbiont, Neorickettsia risticii, during the asexual reproduction phase of its digenean host, Plagiorchis elegans, within naturally infected lymnaeid snails.

Authors:  Stephen E Greiman; Vasyl V Tkach; Jefferson A Vaughan
Journal:  Parasit Vectors       Date:  2013-10-22       Impact factor: 3.876

8.  Large scale screening of digeneans for Neorickettsia endosymbionts using real-time PCR reveals new Neorickettsia genotypes, host associations and geographic records.

Authors:  Stephen E Greiman; Vasyl V Tkach; Eric Pulis; Thomas J Fayton; Stephen S Curran
Journal:  PLoS One       Date:  2014-06-09       Impact factor: 3.240

9.  Serological survey of Ehrlichia species in dogs, horses and humans: zoonotic scenery in a rural settlement from southern Brazil.

Authors:  Rafael Felipe da Costa Vieira; Thállitha Samih Wischral Jayme Vieira; Denise do Amaral Gomes Nascimento; Thiago F Martins; Felipe S Krawczak; Marcelo B Labruna; Ramaswamy Chandrashekar; Mary Marcondes; Alexander Welker Biondo; Odilon Vidotto
Journal:  Rev Inst Med Trop Sao Paulo       Date:  2013 Sep-Oct       Impact factor: 1.846
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.