| Literature DB >> 9103472 |
T Tsuji1, K Hamajima, J Fukushima, K Q Xin, N Ishii, I Aoki, Y Ishigatsubo, K Tani, S Kawamoto, Y Nitta, J Miyazaki, W C Koff, T Okubo, K Okuda.
Abstract
Previous investigations have demonstrated that CTL may play an important role in suppressing the disease progression of HIV infection. In this study, we inoculated mice with IL-12 expression plasmid together with plasmid-encoding HIV-1 envelope to enhance CTL activity by activating a Th1-type response. The results of delayed-type hypersensitivity using the footpad swelling response and of CTL activity clearly showed that HIV-1-specific cell-mediated immunity was enhanced by inoculation of the IL-12 expression plasmid. Quantitation of cytokine in the sera of IL-12-inoculated mice revealed that IFN-gamma significantly increased. The enhanced cell-mediated immunity responses were abrogated by combined administration of the IL-12 expression plasmid and neutralizing anti-IFN-gamma Ab. Together, these results suggest that enhanced virus-specific cell-mediated immunity occurred via an endogenously produced IFN-gamma by inoculation of IL-12 expression plasmid.Entities:
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Year: 1997 PMID： 9103472
Source DB: PubMed Journal: J Immunol ISSN： 0022-1767 Impact factor: 5.422