OBJECTIVE: To examine the effect of hyaluronic acid (HA) on the induction of superoxide anion by IL-1 in chondrocytes. MATERIALS AND METHODS: Bovine articular chondrocytes were treated with different concentrations of IL-1. A chemiluminescent probe (L-012) was added to the medium and chemiluminescence detection was used to measure super oxide anion. RESULTS: IL-1 caused induction of superoxide anions in a dose-dependent manner. HA (10-100 micrograms/ml) countered superoxide induction caused by 20 ng/ml of IL-1. CONCLUSIONS: HA can afford protection against cartilage degradation, probably acting as a free-radical scavenger.
OBJECTIVE: To examine the effect of hyaluronic acid (HA) on the induction of superoxide anion by IL-1 in chondrocytes. MATERIALS AND METHODS:Bovine articular chondrocytes were treated with different concentrations of IL-1. A chemiluminescent probe (L-012) was added to the medium and chemiluminescence detection was used to measure super oxide anion. RESULTS: IL-1 caused induction of superoxide anions in a dose-dependent manner. HA (10-100 micrograms/ml) countered superoxide induction caused by 20 ng/ml of IL-1. CONCLUSIONS:HA can afford protection against cartilage degradation, probably acting as a free-radical scavenger.
Authors: Prem Ramakrishnan; Benjamin A Hecht; Douglas R Pedersen; Matthew R Lavery; Jerry Maynard; Joseph A Buckwalter; James A Martin Journal: J Orthop Res Date: 2010-07 Impact factor: 3.494
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