Substance P facilitates memory in goldfish in an appetitively motivated learning task.

The aim of this study was to investigate if the neuropeptide substance P (SP) can improve learning and memory in goldfish (Carassius auratus). Four groups of fish were trained for seven days to find food in one out of two compartments until discrimination was achieved. On the last training day, they were injected (intra-abdominal) with haloperidol or vehicle before the training, and with SP or vehicle immediately after the training session. Each group of fish received either: (1) vehicle+vehicle (n = 18); (2) vehicle + SP, (n = 20, SP 50 mg/kg); (3) haloperidol+ vehicle (n = 15, haloperidol 2 mg/kg); or (4) haloperidol+ SP (n = 14, haloperidol 2 mg/kg, SP 50 mg/kg). Twenty-four hours later, the time spent to find the food was recorded. Reversal training was done for four consecutive days after this post-injection test and the time spent to find the food was recorded again. The results indicate that only the group treated with vehicle + SP needed more time to reach reversal training than control fish (Mann-Whitney U-test, P = 0.0009). It is suggested that SP can enhance memory in fish and that this effect may have a dopaminergic mediation in discrimination learning task.