| Literature DB >> 9094201 |
G Niebch1, B Büchele, J Blome, S Grieb, G Brandt, P Kampa, H H Raffel, M Locher, H O Borbe, I Nubert, I Fleischhauer.
Abstract
A specific plasma level assay for the enantiomers of alpha-lipoic acid is described. It makes use of liquid-liquid extraction, chemical reduction to the dithiol enantiomers, and their precolumn chiral derivatisation with o-phthalaldehyde in the presence of D-phenylalanine. The two diastereomeric derivatives are separated by reversed-phase HPLC with fluorescence detection. The working range of the assay is between 15 ng/ml (lower limit of quantitation) and 1,000 ng/ml for either enantiomer.Entities:
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Year: 1997 PMID： 9094201 DOI： 10.1002/(SICI)1520-636X(1997)9:1<32::AID-CHIR7>3.0.CO;2-I
Source DB: PubMed Journal: Chirality ISSN： 0899-0042 Impact factor: 2.437