Literature DB >> 9092648

Colorimetric detection of immobilised PCR products generated on a solid support.

A K Lockley1, C G Jones, J S Bruce, S J Franklin, R G Bardsley.   

Abstract

By extending functional primers attached to a solid phase and incorporating a digoxigenin label, it is possible to visualise PCR products as discrete spots on specific regions of a solid support after colorimetric detection. The technique has been used for the detection of the point mutation associated with porcine malignant hyperthermia.

Entities:  

Mesh：

Substances：

Year:  1997        PMID： 9092648      PMCID： PMC146568          DOI： 10.1093/nar/25.6.1313

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Detection of specific alleles by using allele-specific primer extension followed by capture on solid support.

Authors:  L Ugozzoli; J M Wahlqvist; A Ehsani; B E Kaplan; R B Wallace
Journal:  Genet Anal Tech Appl       Date:  1992-08

2.  Detection of Escherichia coli heat-stable enterotoxin genes in pig stool specimens by an immobilized, colorimetric, nested polymerase chain reaction.

Authors:  E Hornes; Y Wasteson; O Olsvik
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948

3.  Colourimetric detection of PCR products using the DNA-binding protein TyrR.

Authors:  T Triglia; V P Argyropoulos; B E Davidson; D J Kemp
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971

4.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

5.  An exonuclease-amplification coupled capture technique improves detection of PCR product.

Authors:  B Holloway; D D Erdman; E L Durigon; J J Murtagh
Journal:  Nucleic Acids Res       Date:  1993-08-11       Impact factor: 16.971

6.  Mutagenically separated PCR (MS-PCR): a highly specific one step procedure for easy mutation detection.

Authors:  S Rust; H Funke; G Assmann
Journal:  Nucleic Acids Res       Date:  1993-08-11       Impact factor: 16.971

7.  Use of a DNA-based test for the mutation associated with porcine stress syndrome (malignant hyperthermia) in 10,000 breeding swine.

Authors:  P J O'Brien; H Shen; C R Cory; X Zhang
Journal:  J Am Vet Med Assoc       Date:  1993-09-15       Impact factor: 1.936

8.  Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia.

Authors:  J Fujii; K Otsu; F Zorzato; S de Leon; V K Khanna; J E Weiler; P J O'Brien; D H MacLennan
Journal:  Science       Date:  1991-07-26       Impact factor: 47.728

9.  Single-base mutational analysis of cancer and genetic diseases using membrane bound modified oligonucleotides.

Authors:  Y Zhang; M Y Coyne; S G Will; C H Levenson; E S Kawasaki
Journal:  Nucleic Acids Res       Date:  1991-07-25       Impact factor: 16.971

10.  Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes.

Authors:  R K Saiki; P S Walsh; C H Levenson; H A Erlich
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205
  10 in total
  4 in total

1.  Use of immobilized PCR primers to generate covalently immobilized DNAs for in vitro transcription/translation reactions.

Authors:  J D Andreadis; L A Chrisey
Journal:  Nucleic Acids Res       Date:  2000-01-15       Impact factor: 16.971

2.  Solid-phase translation and RNA-protein fusion: a novel approach for folding quality control and direct immobilization of proteins using anchored mRNA.

Authors:  Manish Biyani; Yuzuru Husimi; Naoto Nemoto
Journal:  Nucleic Acids Res       Date:  2006-10-24       Impact factor: 16.971

3.  DNA analysis with multiplex microarray-enhanced PCR.

Authors:  A Pemov; H Modi; D P Chandler; S Bavykin
Journal:  Nucleic Acids Res       Date:  2005-01-20       Impact factor: 16.971

Review 4.  Integrated Amplification Microarrays for Infectious Disease Diagnostics.

Authors:  Darrell P Chandler; Lexi Bryant; Sara B Griesemer; Rui Gu; Christopher Knickerbocker; Alexander Kukhtin; Jennifer Parker; Cynthia Zimmerman; Kirsten St George; Christopher G Cooney
Journal:  Microarrays (Basel)       Date:  2012-11-09
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.