[The cholinesterase reactivity of the Komandor squid Berryteuthis magister. Substrates and organophosphorus inhibitors].

With the substrate inhibition analysis it has been shown that the one enzyme is responsible for the choline-esterase activity of optic ganglia tissue of Comandor squid Berryteuthis magister from different regions of Bering and Japanese seas. The kinetic parameters of the choline-esterase hydrolysis with 8 choline esters have been determined. It demonstrates the wide spectrum of the substrate specificity of Comandor squid choline-esterase. The antienzyme efficiency for 18 organophosphorus inhibitors (among them 7 compounds containing acetylene group) has been investigated. Also the new information about choline-esterase "structure-action" connection for Comandor squid has been obtained. The quantitative differences in the substrate specificity and reactivity for organophosphorus inhibitors of choline-esterases of Comandor and Pacific squids have been obtained. It has not succeeded to find the intraspecific differences for Comandor squid species from various living zones using the investigated effects.