S Ma1, D K Kalousek, B H Yuen, Y S Moon. 1. Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, Canada.
Abstract
PURPOSE: The objective of this study was to examine the effect of superovulatory doses of gonadotropins on the frequency of chromosomal abnormalities of mouse embryos. METHODS: Chromosome analysis of 8- to 16-cell stage mouse embryos and zygotes was performed by a cytogenetic method. RESULTS: There was no significant effect of the pregnant mare serum gonadotropin (PMSG) dose on the level of aneuploidy and structural abnormalities from 8- to 16-cell-stage embryos among superovulated groups. However, a simple dose-response relationship between the PMSG dose and the incidence of polyploidy was observed, with the level of polyploidy rising from 2.9% with 10 i.u. PMSG to 10.5% with 15 i.u. PMSG. In zygote stage, the proportion of polyploid embryos also increased as the dose increased, from 1.9% in 5 i.u. to 6.7% in 15 i.u. PMSG. It was observed that the extra chromosomal set in polyploidy embryos originated by both fertilization of a diploid oocyte and dispermy. CONCLUSIONS: These results indicate a dose-response relationship between the PMSG dose and the incidence of polyploidy in the CD-1 mouse. Both a disturbance at maturation division and an error at fertilization were the cause of polyploidy.
PURPOSE: The objective of this study was to examine the effect of superovulatory doses of gonadotropins on the frequency of chromosomal abnormalities of mouse embryos. METHODS: Chromosome analysis of 8- to 16-cell stage mouse embryos and zygotes was performed by a cytogenetic method. RESULTS: There was no significant effect of the pregnant mare serum gonadotropin (PMSG) dose on the level of aneuploidy and structural abnormalities from 8- to 16-cell-stage embryos among superovulated groups. However, a simple dose-response relationship between the PMSG dose and the incidence of polyploidy was observed, with the level of polyploidy rising from 2.9% with 10 i.u. PMSG to 10.5% with 15 i.u. PMSG. In zygote stage, the proportion of polyploid embryos also increased as the dose increased, from 1.9% in 5 i.u. to 6.7% in 15 i.u. PMSG. It was observed that the extra chromosomal set in polyploidy embryos originated by both fertilization of a diploid oocyte and dispermy. CONCLUSIONS: These results indicate a dose-response relationship between the PMSG dose and the incidence of polyploidy in the CD-1mouse. Both a disturbance at maturation division and an error at fertilization were the cause of polyploidy.
Authors: M H Pieters; J C Dumoulin; R C Ignoul-Vanvuchelen; M Bras; J L Evers; J P Geraedts Journal: J Assist Reprod Genet Date: 1992-02 Impact factor: 3.412
Authors: Ahmed M Taiyeb; Saeeda A Muhsen-Alanssari; W L Dees; Mundhir T Ridha-Albarzanchi; Duane C Kraemer Journal: Exp Biol Med (Maywood) Date: 2014-09-21