The effect of dietary iron deficiency on the fatty acid composition of plasma and erythrocyte membrane phospholipids in the rat.

Severe iron deficiency was introduced in rats by feeding outbred male Wistar rats a purified diet that was either adequate or deficient in iron. The rats were weighed regularly over 4 weeks to monitor body weight differences, after which blood was drawn from a subsample to determine the haemoglobin concentrations and fatty acid composition of plasma total phospholipids and to measure the erythrocyte membrane phosphatidylcholine and phosphatidylethanolamine levels. Comparisons between dietary iron adequate (control) and dietary iron deficient (experimental) rats showed that the experimental rats had lower body weight and plasma total phospholipid linoleic acid levels typical of the symptoms of essential fatty acid deficiency. Erythrocyte membrane phosphatidylethanolamine arachidonic acid levels were increased (P < 0.05) with concomitant decreases in oleic acid (P < 0.01). Correlations between fatty acids and growth suggest that the mechanism whereby iron deficiency affects growth is in some way related to abnormal fatty acid shifts that disturb the delicate balance of essential fatty acids in membranes. Additional omega 3 and omega 6 fatty acids may be necessary to counteract the effect of iron deficiency in rats.