BACKGROUND AND OBJECTIVES: Serotyping of antibody to hepatitis C virus (anti-HCV) with specific peptides has been developed as an alternate method for typing HCV infections. The method does not require a prior amplification of viral RNA sequences from the sample. Identification of the viral genotype may be relevant for prognosis and clinical management. MATERIALS AND METHODS: We used a previously described HCV serotyping assay (RIBA HCV Serotype SIA kit, Chiron Corp.) to investigate the serotype in 191 samples from blood donors selected for anti-HCV patterns (positive and indeterminate), ALT levels, and the presence or absence of viral RNA. The serotypes were compared with the genotypes obtained from typing the 5'-noncoding region of the viral RNA in 82 viremic samples. RESULTS: We were able to obtain the viral serotype in 85% (114/134) of samples positive for anti-HCV but in only 3.5% (2/57) of the indeterminates. Lack of anti-NS4 in the sample was significantly associated with both untypable results and the presence of HCV serotypes other than serotype 1. The overall correlation with genotyping was 78% (64/82), rising to 95.5% (64/67) if only samples that could be both genotyped and serotyped were considered. The assay was easy to perform, gave reactivity patterns easy to interpret, and performed with high proficiency on anti-HCV-positive samples lacking detectable levels of viral RNA. CONCLUSIONS: This is a practical and useful method for typing HCV infections in the clinical setting. The poor ability of the Core peptides to give the serotype in samples lacking anti-NS4 and the lack of specific peptides to recognize HCV types other than 1, 2, and 3 are, however, some aspects of the method that need improvement in the future.
BACKGROUND AND OBJECTIVES: Serotyping of antibody to hepatitis C virus (anti-HCV) with specific peptides has been developed as an alternate method for typing HCV infections. The method does not require a prior amplification of viral RNA sequences from the sample. Identification of the viral genotype may be relevant for prognosis and clinical management. MATERIALS AND METHODS: We used a previously described HCV serotyping assay (RIBA HCV Serotype SIA kit, Chiron Corp.) to investigate the serotype in 191 samples from blood donors selected for anti-HCV patterns (positive and indeterminate), ALT levels, and the presence or absence of viral RNA. The serotypes were compared with the genotypes obtained from typing the 5'-noncoding region of the viral RNA in 82 viremic samples. RESULTS: We were able to obtain the viral serotype in 85% (114/134) of samples positive for anti-HCV but in only 3.5% (2/57) of the indeterminates. Lack of anti-NS4 in the sample was significantly associated with both untypable results and the presence of HCV serotypes other than serotype 1. The overall correlation with genotyping was 78% (64/82), rising to 95.5% (64/67) if only samples that could be both genotyped and serotyped were considered. The assay was easy to perform, gave reactivity patterns easy to interpret, and performed with high proficiency on anti-HCV-positive samples lacking detectable levels of viral RNA. CONCLUSIONS: This is a practical and useful method for typing HCV infections in the clinical setting. The poor ability of the Core peptides to give the serotype in samples lacking anti-NS4 and the lack of specific peptides to recognize HCV types other than 1, 2, and 3 are, however, some aspects of the method that need improvement in the future.
Authors: P Dentico; N Curatolo; R Sacco; M De Luca; A Volpe; C Ranieri; C Genchi; L Petracca; R Buongiorno Journal: Infection Date: 1999 Mar-Apr Impact factor: 3.553
Authors: M Beld; M Penning; M van Putten; A van den Hoek; V Lukashov; M McMorrow; J Goudsmit Journal: J Clin Microbiol Date: 1998-10 Impact factor: 5.948