Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Sizing membrane pores in lipid vesicles by leakage of co-encapsulated markers: pore formation by melittin.

Literature DB >> 9083680

Sizing membrane pores in lipid vesicles by leakage of co-encapsulated markers: pore formation by melittin.

Abstract

Many toxins and antimicrobial peptides permeabilize membrane vesicles by forming multimeric pores. Determination of the size of such pores is an important first step for understanding their structure and the mechanism of their self-assembly. We report a simple method for sizing pores in vesicles based on the differential release of co-encapsulated fluorescently labeled dextran markers of two different sizes. The method was tested using the bee venom peptide melittin, which was found to form pores of 25-30 A diameter in palmitoyloleoylphosphatidylcholine (POPC) vesicles at a lipid-to-peptide ratio of 50. This result is consistent with observations on melittin pore formation in erythrocytes (Katsu, T., C. Ninomiya, M. Kuroko, H. Kobayashi, T. Hirota, and Y. Fujita 1988. Action mechanism of amphipathic peptides gramicidin S and melittin on erythrocyte membrane Biochim. Biophys. Acta. 939:57-63).

Entities: Chemical

Mesh：

Substances：

Year: 1997 PMID： 9083680 PMCID： PMC1184370 DOI： 10.1016/S0006-3495(97)78822-2

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

21 in total

1. Kinetics of melittin induced pore formation in the membrane of lipid vesicles.

Authors: G Schwarz; R T Zong; T Popescu
Journal: Biochim Biophys Acta Date: 1992-09-21

2. Mechanism of leakage of phospholipid vesicle contents induced by the peptide GALA.

Authors: R A Parente; S Nir; F C Szoka
Journal: Biochemistry Date: 1990-09-18 Impact factor: 3.162

3. Release of lipid vesicle contents by the bacterial protein toxin alpha-haemolysin.

Authors: H Ostolaza; B Bartolomé; I Ortiz de Zárate; F de la Cruz; F M Goñi
Journal: Biochim Biophys Acta Date: 1993-04-08

Review 4. The actions of melittin on membranes.

Authors: C E Dempsey
Journal: Biochim Biophys Acta Date: 1990-05-07

5. [The effect of anions on the hemolytic activity of melittin].

Authors: R Z Sabirov; O V Krasil'nikov; S V Merkulova; E G Kostrzhevskaia; N V Shcherbatskaia
Journal: Ukr Biokhim Zh (1978) Date: 1990 Jan-Feb

6. Melittin lysis of red cells.

Authors: M T Tosteson; S J Holmes; M Razin; D C Tosteson
Journal: J Membr Biol Date: 1985 Impact factor: 1.843

7. Kinetics and mechanism of hemolysis induced by melittin and by a synthetic melittin analogue.

Authors: W F DeGrado; G F Musso; M Lieber; E T Kaiser; F J Kézdy
Journal: Biophys J Date: 1982-01 Impact factor: 4.033

8. Action mechanism of amphipathic peptides gramicidin S and melittin on erythrocyte membrane.

Authors: T Katsu; C Ninomiya; M Kuroko; H Kobayashi; T Hirota; Y Fujita
Journal: Biochim Biophys Acta Date: 1988-03-22

9. Morphological changes of phosphatidylcholine bilayers induced by melittin: vesicularization, fusion, discoidal particles.

Authors: J Dufourcq; J F Faucon; G Fourche; J L Dasseux; M Le Maire; T Gulik-Krzywicki
Journal: Biochim Biophys Acta Date: 1986-07-10

10. Quantitative description of the absorption spectra of the coenzyme in glycogen phosphorylases based on log-normal distribution curves.

Authors: J Donoso; F Muñoz; F Garcia Blanco
Journal: Biochem J Date: 1993-05-15 Impact factor: 3.857

65 in total

Sizing membrane pores in lipid vesicles by leakage of co-encapsulated markers: pore formation by melittin.

1. Kinetics of melittin induced pore formation in the membrane of lipid vesicles.

2. Mechanism of leakage of phospholipid vesicle contents induced by the peptide GALA.

3. Release of lipid vesicle contents by the bacterial protein toxin alpha-haemolysin.

Review 4. The actions of melittin on membranes.

5. [The effect of anions on the hemolytic activity of melittin].

6. Melittin lysis of red cells.

7. Kinetics and mechanism of hemolysis induced by melittin and by a synthetic melittin analogue.

8. Action mechanism of amphipathic peptides gramicidin S and melittin on erythrocyte membrane.

9. Morphological changes of phosphatidylcholine bilayers induced by melittin: vesicularization, fusion, discoidal particles.

10. Quantitative description of the absorption spectra of the coenzyme in glycogen phosphorylases based on log-normal distribution curves.

1. Structure, location, and lipid perturbations of melittin at the membrane interface.

2. Supramolecular structures of peptide assemblies in membranes by neutron off-plane scattering: method of analysis.

3. Barrel-stave model or toroidal model? A case study on melittin pores.

4. Insertion and pore formation driven by adsorption of proteins onto lipid bilayer membrane-water interfaces.

5. Energetics and self-assembly of amphipathic peptide pores in lipid membranes.

6. Effect of phospholipid composition on an amphipathic peptide-mediated pore formation in bilayer vesicles.

7. Structure of (KIAGKIA)3 aggregates in phospholipid bilayers by solid-state NMR.

8. The dynamics of melittin-induced membrane permeability.

9. The electrical response of bilayers to the bee venom toxin melittin: evidence for transient bilayer permeabilization.

10. Mixtures of supported and hybrid lipid membranes on heterogeneously modified silica nanoparticles.