| Literature DB >> 9080328 |
D Germadnik1, A Pilger, H W Rüdiger.
Abstract
A high-performance liquid chromatographic procedure with electrochemical detection is described for the determination of urinary 8-hydroxy-2'-deoxyguanosine, a major oxidative DNA lesion induced by radical forming agents. A two-step solid-phase extraction procedure was followed for extracting 8-hydroxy-2'-deoxyguanosine from human urine and the analysis was performed on a RP-18 analytical column under isocratic conditions. The limit of detection of 8-hydroxy-2'-deoxyguanosine in urine was found to be 0.9 nM. The non-invasive assay provides an indirect measurement of oxidative DNA damage.Entities:
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Year: 1997 PMID： 9080328 DOI： 10.1016/s0378-4347(96)00328-3
Source DB: PubMed Journal: J Chromatogr B Biomed Sci Appl ISSN： 1387-2273