Literature DB >> 9067975

Phage-display libraries of murine and human antibody Fab fragments.

J Engberg1, P S Andersen, L K Nielsen, M Dziegiel, L K Johansen, B Albrechtsen.   

Abstract

We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

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Year:  1996        PMID： 9067975     DOI： 10.1007/BF02761708

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  16 in total

1.  Expression of functional antibody Fv and Fab fragments in Escherichia coli.

Authors:  A Plückthun; A Skerra
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

2.  A surface expression vector for antibody screening.

Authors:  F Breitling; S Dübel; T Seehaus; I Klewinghaus; M Little
Journal:  Gene       Date:  1991-08-15       Impact factor: 3.688

3.  Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains.

Authors:  H R Hoogenboom; A D Griffiths; K S Johnson; D J Chiswell; P Hudson; G Winter
Journal:  Nucleic Acids Res       Date:  1991-08-11       Impact factor: 16.971

4.  One-step cloning of murine Fab gene fragments independent of IgH isotype for phage display libraries.

Authors:  P S Andersen; H Orum; J Engberg
Journal:  Biotechniques       Date:  1996-03       Impact factor: 1.993

5.  pFab60: a new, efficient vector for expression of antibody Fab fragments displayed on phage.

Authors:  L K Johansen; B Albrechtsen; H W Andersen; J Engberg
Journal:  Protein Eng       Date:  1995-10

6.  High efficiency transformation of E. coli by high voltage electroporation.

Authors:  W J Dower; J F Miller; C W Ragsdale
Journal:  Nucleic Acids Res       Date:  1988-07-11       Impact factor: 16.971

7.  Growth of E. coli at low temperature dramatically increases the transformation frequency by electroporation.

Authors:  S E Chuang; A L Chen; C C Chao
Journal:  Nucleic Acids Res       Date:  1995-05-11       Impact factor: 16.971

Review 8.  Making antibodies by phage display technology.

Authors:  G Winter; A D Griffiths; R E Hawkins; H R Hoogenboom
Journal:  Annu Rev Immunol       Date:  1994       Impact factor: 28.527

9.  Two-step cloning of antibody variable domains in a phage display vector.

Authors:  O Engelhardt; R Grabherr; G Himmler; F Rüker
Journal:  Biotechniques       Date:  1994-07       Impact factor: 1.993

10.  Spliceosomal small nuclear RNAs of Tetrahymena thermophila and some possible snRNA-snRNA base-pairing interactions.

Authors:  H Orum; H Nielsen; J Engberg
Journal:  J Mol Biol       Date:  1991-11-20       Impact factor: 5.469
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  4 in total

1.  Human recombinant antibodies against Plasmodium falciparum merozoite surface protein 3 cloned from peripheral blood leukocytes of individuals with immunity to malaria demonstrate antiparasitic properties.

Authors:  Rasmus Lundquist; Leif Kofoed Nielsen; Ali Jafarshad; Daw Soesoe; Lars Harder Christensen; Pierre Druilhe; Morten Hanefeld Dziegiel
Journal:  Infect Immun       Date:  2006-06       Impact factor: 3.441

Review 2.  Phage displayed peptides/antibodies recognizing growth factors and their tyrosine kinase receptors as tools for anti-cancer therapeutics.

Authors:  Roberto Ronca; Patrizia Benzoni; Angela De Luca; Elisabetta Crescini; Patrizia Dell'Era
Journal:  Int J Mol Sci       Date:  2012-04-24       Impact factor: 6.208

3.  Proteomic analysis and discovery using affinity proteomics and mass spectrometry.

Authors:  Niclas Olsson; Christer Wingren; Mikael Mattsson; Peter James; David O'Connell; Fredrik Nilsson; Dolores J Cahill; Carl A K Borrebaeck
Journal:  Mol Cell Proteomics       Date:  2011-06-14       Impact factor: 5.911

4.  Visualization of myelin basic protein (MBP) T cell epitopes in multiple sclerosis lesions using a monoclonal antibody specific for the human histocompatibility leukocyte antigen (HLA)-DR2-MBP 85-99 complex.

Authors:  M Krogsgaard; K W Wucherpfennig; B Cannella; B E Hansen; A Svejgaard; J Pyrdol; H Ditzel; C Raine; J Engberg; L Fugger; B Canella
Journal:  J Exp Med       Date:  2000-04-17       Impact factor: 14.307
  4 in total

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