Literature DB >> 9067016

"Megaprimer" method of PCR-based mutagenesis: the concentration of megaprimer is a critical factor.

A M Smith1, K P Klugman.   

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Year:  1997        PMID： 9067016     DOI： 10.2144/97223bm13

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


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  12 in total

1.  Function of the central domain of streptokinase in substrate plasminogen docking and processing revealed by site-directed mutagenesis.

Authors:  A Chaudhary; S Vasudha; K Rajagopal; S S Komath; N Garg; M Yadav; S C Mande; G Sahni
Journal:  Protein Sci       Date:  1999-12       Impact factor: 6.725

2.  Alterations in PBP 1A essential-for high-level penicillin resistance in Streptococcus pneumoniae.

Authors:  A M Smith; K P Klugman
Journal:  Antimicrob Agents Chemother       Date:  1998-06       Impact factor: 5.191

3.  Mutations in ribosomal protein L16 conferring reduced susceptibility to evernimicin (SCH27899): implications for mechanism of action.

Authors:  P V Adrian; W Zhao; T A Black; K J Shaw; R S Hare; K P Klugman
Journal:  Antimicrob Agents Chemother       Date:  2000-03       Impact factor: 5.191

4.  Mutations in the dihydrofolate reductase gene of trimethoprim-resistant isolates of Streptococcus pneumoniae.

Authors:  P V Adrian; K P Klugman
Journal:  Antimicrob Agents Chemother       Date:  1997-11       Impact factor: 5.191

5.  Essential roles for imuA'- and imuB-encoded accessory factors in DnaE2-dependent mutagenesis in Mycobacterium tuberculosis.

Authors:  Digby F Warner; Duduzile E Ndwandwe; Garth L Abrahams; Bavesh D Kana; Edith E Machowski; Ceslovas Venclovas; Valerie Mizrahi
Journal:  Proc Natl Acad Sci U S A       Date:  2010-07-06       Impact factor: 11.205

6.  Resuscitation-Promoting Factors Are Required for Mycobacterium smegmatis Biofilm Formation.

Authors:  Christopher Ealand; Binayak Rimal; James Chang; Lethabo Mashigo; Melissa Chengalroyen; Lusanda Mapela; Germar Beukes; Edith Machowski; Sung Joon Kim; Bavesh Kana
Journal:  Appl Environ Microbiol       Date:  2018-08-17       Impact factor: 4.792

7.  Role of penicillin-binding protein 5 in expression of ampicillin resistance and peptidoglycan structure in Enterococcus faecium.

Authors:  F Sifaoui; M Arthur; L Rice; L Gutmann
Journal:  Antimicrob Agents Chemother       Date:  2001-09       Impact factor: 5.191

8.  An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes.

Authors:  R D Kirsch; E Joly
Journal:  Nucleic Acids Res       Date:  1998-04-01       Impact factor: 16.971

9.  Evernimicin (SCH27899) inhibits a novel ribosome target site: analysis of 23S ribosomal DNA mutants.

Authors:  P V Adrian; C Mendrick; D Loebenberg; P McNicholas; K J Shaw; K P Klugman; R S Hare; T A Black
Journal:  Antimicrob Agents Chemother       Date:  2000-11       Impact factor: 5.191

10.  ATP-bound conformation of topoisomerase IV: a possible target for quinolones in Streptococcus pneumoniae.

Authors:  Farid Sifaoui; Valérie Lamour; Emmanuelle Varon; Dino Moras; Laurent Gutmann
Journal:  J Bacteriol       Date:  2003-10       Impact factor: 3.490
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