| Literature DB >> 9065694 |
D Prüfer1, J Schmitz, E Tacke, B Kull, W Rohde.
Abstract
A full-length cDNA copy (PLRVfl) of potato leafroll virus (PLRV) was constructed and examined in vivo for its biological activities by transient expression experiments with plasmid DNA or in vitro transcribed RNA. In addition, PLRVfl cDNA was stably introduced into the genome of potato plants by Agrobacterium-mediated leaf disc transformation. Both transient and stable expression of PLRVfl resulted in the synthesis of genomic and subgenomic PLRV RNAs. Transgenic plants accumulated the 17-kDa movement protein and displayed the typical symptoms of PLRV infection. This is the first example of the constitutive expression of a phloem-limited virus in planta.Entities:
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Year: 1997 PMID： 9065694 DOI： 10.1007/s004380050363
Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925