OBJECTIVES: This study was undertaken to determine whether tissue-specific defensins are expressed within female reproductive tissues. STUDY DESIGN: Messenger ribonucleic acid from amnion, chorion, endometrium, endocervix, myometrium, placenta, small intestine, peripheral blood lymphocytes, and cervical, endometrial, and trophoblast cell lines was reverse transcribed with a 3'-RACE adapter. 3'-RACE polymerase chain reaction was conducted with an upstream human defensin 5 primer and 3'-RACE adapter primer. Polymerase chain reaction products hybridizing to a human defensin 5 probe were cloned for sequence analysis. Sequence data were compared against a nucleotide sequence database, and secondary structure predictions were made. RESULTS: Chorionic tissue, endocervical tissue, endometrial tissue, and an endometrial cell line all demonstrated a single hybridizing 362 bp polymerase chain reaction product. Sequence analysis of all clones demonstrated near-perfect identity with human defensin 5. CONCLUSIONS: Human endocervix, endometrium, and chorion express defensin 5 at the level of transcription. These findings suggest that a previously unrecognized mechanism of protecting female reproductive tissues against infection, by means of a natural antimicrobial system (defensins), may be present.
OBJECTIVES: This study was undertaken to determine whether tissue-specific defensins are expressed within female reproductive tissues. STUDY DESIGN: Messenger ribonucleic acid from amnion, chorion, endometrium, endocervix, myometrium, placenta, small intestine, peripheral blood lymphocytes, and cervical, endometrial, and trophoblast cell lines was reverse transcribed with a 3'-RACE adapter. 3'-RACE polymerase chain reaction was conducted with an upstream humandefensin 5 primer and 3'-RACE adapter primer. Polymerase chain reaction products hybridizing to a humandefensin 5 probe were cloned for sequence analysis. Sequence data were compared against a nucleotide sequence database, and secondary structure predictions were made. RESULTS: Chorionic tissue, endocervical tissue, endometrial tissue, and an endometrial cell line all demonstrated a single hybridizing 362 bp polymerase chain reaction product. Sequence analysis of all clones demonstrated near-perfect identity with humandefensin 5. CONCLUSIONS:Human endocervix, endometrium, and chorion express defensin 5 at the level of transcription. These findings suggest that a previously unrecognized mechanism of protecting female reproductive tissues against infection, by means of a natural antimicrobial system (defensins), may be present.
Authors: Lyndsey R Buckner; Danny J Schust; Jian Ding; Takeshi Nagamatsu; Wandy Beatty; Theresa L Chang; Sheila J Greene; Maria E Lewis; Bernardo Ruiz; Stacey L Holman; Rae Ann Spagnuolo; Richard B Pyles; Alison J Quayle Journal: J Reprod Immunol Date: 2011-09-22 Impact factor: 4.054
Authors: R Romero; J P Kusanovic; J Espinoza; F Gotsch; C L Nhan-Chang; O Erez; C J Kim; N Khalek; P Mittal; L F Goncalves; C Schaudinn; S S Hassan; J W Costerton Journal: Ultrasound Obstet Gynecol Date: 2007-10 Impact factor: 7.299
Authors: Roberto Romero; Lara A Friel; Digna R Velez Edwards; Juan Pedro Kusanovic; Sonia S Hassan; Shali Mazaki-Tovi; Edi Vaisbuch; Chong Jai Kim; Offer Erez; Tinnakorn Chaiworapongsa; Brad D Pearce; Jacquelaine Bartlett; Benjamin A Salisbury; Madan Kumar Anant; Gerald F Vovis; Min Seob Lee; Ricardo Gomez; Ernesto Behnke; Enrique Oyarzun; Gerard Tromp; Scott M Williams; Ramkumar Menon Journal: Am J Obstet Gynecol Date: 2010-07-31 Impact factor: 8.661
Authors: Francesca Gotsch; Roberto Romero; Jimmy Espinoza; Juan Pedro Kusanovic; Shali Mazaki-Tovi; Offer Erez; Nandor Gabor Than; Samuel Edwin; Moshe Mazor; Bo Hyan Yoon; Sonia S Hassan Journal: J Matern Fetal Neonatal Med Date: 2007-10
Authors: Carole L Wilson; Amy P Schmidt; Emma Pirilä; Erika V Valore; Nicola Ferri; Timo Sorsa; Tomas Ganz; William C Parks Journal: J Biol Chem Date: 2009-01-30 Impact factor: 5.157