Effect of glutathione, catechin, and epicatechin on the survival of Drosophila melanogaster under paraquat treatment.

The biological effect of antioxidants which showed high superoxide-scavenging (SOS) activity in an in vitro analysis was examined by using Drosophila melanogaster. When the flies were exposed to paraquat as an endogenous source of the superoxide anion, their survival rapidly decreased. Although the SOS antioxidants did not have a preventive effect against paraquat toxicity, a supplement of each SOS antioxidants as glutathione, (+)-catechin and/or (-)-epicatechin to the diet had a reparative effect on flies damaged by the superoxide anion. The survival ratio of flies fed on a diet enriched with SOS antioxidants ranged from 77% to 87%, while that of the control group was 56%. When flies were exposed to paraquat in the presence of hydrogen peroxide or iron, each combination was more toxic than paraquat alone, since the two compounds could accelerate the generation of reactive oxygen species in vivo. The SOS antioxidants, however, allowed the flies to resist the combined toxicity of paraquat and ferrous iron.