Literature DB >> 9056269

Stimulation of calcium influx and platelet activation by canatoxin: methoxyverapamil inhibition and downregulation by cGMP.

F A Ghazaleh1, I M Francischetti, M E Gombarovits, C R Carlini.   

Abstract

Canatoxin (CNTX), a toxic protein isolated from seeds of Canavalia ensiformis, induces Ca2+ influx across the platelet plasma membrane, mobilization of arachidonic acid mediated by phospholipase A2, ATP secretion, and platelet aggregation. All these events depend on the presence of extracellular Ca2+ and are blocked by methoxyverapamil, a calcium-channel blocker. CNTX does not activate phospholipase C, and the intracellular calcium mobilization mediated by IP3 does not play a role in platelet activation by this toxin. Preincubation of rabbit platelets with 8-Br-cGMP inhibited the CNTX-evoked calcium influx, arachidonate release, ATP secretion, and cell aggregation. Our data suggest that the calcium influx is a prior step on platelet activation by CNTX, being modulated by cGMP.

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Year:  1997        PMID： 9056269     DOI： 10.1006/abbi.1997.9898

Source DB:  PubMed          Journal:  Arch Biochem Biophys        ISSN： 0003-9861            Impact factor:   4.013


  7 in total

1.  Canatoxin, a toxic protein from jack beans (Canavalia ensiformis), is a variant form of urease (EC 3.5.1.5): biological effects of urease independent of its ureolytic activity.

Authors:  C Follmer; G B Barcellos; R B Zingali; O L Machado; E W Alves; C Barja-Fidalgo; J A Guimarães; C R Carlini
Journal:  Biochem J       Date:  2001-11-15       Impact factor: 3.857

2.  Proteus mirabilis Urease: Unsuspected Non-Enzymatic Properties Relevant to Pathogenicity.

Authors:  Matheus V C Grahl; Augusto F Uberti; Valquiria Broll; Paula Bacaicoa-Caruso; Evelin F Meirelles; Celia R Carlini
Journal:  Int J Mol Sci       Date:  2021-07-04       Impact factor: 5.923

3.  The Impact of Helicobacter pylori Urease upon Platelets and Consequent Contributions to Inflammation.

Authors:  Adriele Scopel-Guerra; Deiber Olivera-Severo; Fernanda Staniscuaski; Augusto F Uberti; Natália Callai-Silva; Natália Jaeger; Bárbara N Porto; Celia R Carlini
Journal:  Front Microbiol       Date:  2017-12-12       Impact factor: 5.640

Review 4.  Ureases: Historical aspects, catalytic, and non-catalytic properties - A review.

Authors:  Karine Kappaun; Angela Regina Piovesan; Celia Regina Carlini; Rodrigo Ligabue-Braun
Journal:  J Adv Res       Date:  2018-05-28       Impact factor: 10.479

5.  Helicobacter pylori urease activates blood platelets through a lipoxygenase-mediated pathway.

Authors:  German E Wassermann; Deiber Olivera-Severo; Augusto F Uberti; Célia R Carlini
Journal:  J Cell Mol Med       Date:  2009-09-14       Impact factor: 5.310

6.  Humoral and cellular immune responses induced by the urease-derived peptide Jaburetox in the model organism Rhodnius prolixus.

Authors:  Leonardo L Fruttero; Natalia R Moyetta; Augusto F Uberti; Matheus V Coste Grahl; Fernanda C Lopes; Valquiria Broll; Denise Feder; Celia R Carlini
Journal:  Parasit Vectors       Date:  2016-07-25       Impact factor: 3.876

7.  Helicobacter pylori Urease: Potential Contributions to Alzheimer's Disease.

Authors:  Augusto F Uberti; Natalia Callai-Silva; Matheus V C Grahl; Angela R Piovesan; Eduarda G Nachtigall; Cristiane R G Furini; Celia Regina Carlini
Journal:  Int J Mol Sci       Date:  2022-03-13       Impact factor: 5.923
  7 in total

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