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Literature DB >> 903964

Comparison between the size of granular vesicles in intact cells and vesicles obtained by fragmentation of biomembranes.

Abstract

Preparations of biomembranes, consisting of membrane vesicles, were analyzed with the analytical ultracentrifuge. Under certain conditions depending on the speed of rotation and the temperature, a sedimentation profile was observed that was highly characteristic for membranous material. From the sedimentation coefficients obtained, we calculated particle weights for the various well-defined membrane components. In certain types of preparations the particle weights of two adjacent components differed on average by a factor of 2. When vesicles obtained by fragmentation of biomembranes were compared with the granular vesicles present in intact cells, the accordance in diameters was striking. This may indicate that the size of vesicles is determined by purely physical factors.

Mesh：

Substances：
Liposomes

Year: 1977 PMID： 903964 DOI： 10.1007/bf01868152

Source DB: PubMed Journal: J Membr Biol ISSN： 0022-2631 Impact factor: 1.843

13 in total

1. NEGATIVE STAINING OF PHOSPHOLIPIDS AND THEIR STRUCTURAL MODIFICATION BY SURFACE-ACTIVE AGENTS AS OBSERVED IN THE ELECTRON MICROSCOPE.

Authors: A D BANGHAM; R W HORNE
Journal: J Mol Biol Date: 1964-05 Impact factor: 5.469

2. Sedimentation studies on membrane vesicles.

Authors: J Boom; W S Bont; H P Hofs; M De Vries
Journal: Mol Biol Rep Date: 1976-09 Impact factor: 2.316

3. Isolation of plasma membranes from rat and mouse livers and hepatomas.

Authors: P Emmelot; C J Bos; R P van Hoeven; W J van Blitterswijk
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

4. Studies on plasma membranes. 8. The effects of sodium deoxycholate and dodecyl sulfate on isolated rat-liver plasma membranes.

Authors: W S Bont; P Emmelot; H Vaz Dias
Journal: Biochim Biophys Acta Date: 1969-04

Comparison between the size of granular vesicles in intact cells and vesicles obtained by fragmentation of biomembranes.

1. NEGATIVE STAINING OF PHOSPHOLIPIDS AND THEIR STRUCTURAL MODIFICATION BY SURFACE-ACTIVE AGENTS AS OBSERVED IN THE ELECTRON MICROSCOPE.

2. Sedimentation studies on membrane vesicles.

3. Isolation of plasma membranes from rat and mouse livers and hepatomas.

4. Studies on plasma membranes. 8. The effects of sodium deoxycholate and dodecyl sulfate on isolated rat-liver plasma membranes.

5. Lipid composition and permeability of liposomes.

6. Distribution of granular vesicles in normal and constricted sympathetic neurones.

7. Studies on plasma membranes. XIX. Isolation and characterization of a plasma membrane fraction from calf thymocytes.

8. Studies on the endoplasmic reticulum. II. Simple dispositions in cells in situ.

9. AN ELECTRON MICROSCOPE STUDY OF CULTURED RAT SPINAL CORD.

10. Isolation and biological properties of secretory granules from rat anterior pituitary glands.

1. Membrane vesicles of cellular dimensions fit in two geometric series.

2. Localization of Aplysia neurosecretory peptides to multiple populations of dense core vesicles.