Literature DB >> 9032315

The NPI-1/NPI-3 (karyopherin alpha) binding site on the influenza a virus nucleoprotein NP is a nonconventional nuclear localization signal.

P Wang1, P Palese, R E O'Neill.   

Abstract

Two cellular proteins, NPI-1 and NPI-3, were previously identified through their interaction with the influenza virus nucleoprotein (NP) by using the yeast two-hybrid system. These proteins were then shown to act as general transport factors (karyopherin alpha) and nuclear pore-docking proteins to facilitate the transport of the NP and of viral RNA into the nucleus. The yeast two-hybrid assay has now been used to identify the specific domains on the NP that bind to the NPI proteins. Mutational analysis including alanine scanning identified the motifs SxGTKRSYxxM and TKRSxxxM, which are required for binding to NPI-1 and NPI-3, respectively. These sequences were shown to possess nuclear localization signal (NLS) activity following expression of fusion proteins in HeLa cells. These sequences represent a novel nonconventional NLS motif. Another NLS activity not mediated by the NPI binding sites is associated with noncontiguous sequences in the NP.

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Year:  1997        PMID： 9032315      PMCID： PMC191255     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  27 in total

1.  Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library.

Authors:  M D Adams; M B Soares; A R Kerlavage; C Fields; J C Venter
Journal:  Nat Genet       Date:  1993-08       Impact factor: 38.330

2.  Nuclear import of influenza virus RNA can be mediated by viral nucleoprotein and transport factors required for protein import.

Authors:  R E O'Neill; R Jaskunas; G Blobel; P Palese; J Moroianu
Journal:  J Biol Chem       Date:  1995-09-29       Impact factor: 5.157

3.  Transformation of intact yeast cells treated with alkali cations.

Authors:  H Ito; Y Fukuda; K Murata; A Kimura
Journal:  J Bacteriol       Date:  1983-01       Impact factor: 3.490

4.  Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal and beta subunit interacts with peptide repeat-containing nucleoporins.

Authors:  J Moroianu; M Hijikata; G Blobel; A Radu
Journal:  Proc Natl Acad Sci U S A       Date:  1995-07-03       Impact factor: 11.205

5.  Pendulin, a Drosophila protein with cell cycle-dependent nuclear localization, is required for normal cell proliferation.

Authors:  P Küssel; M Frasch
Journal:  J Cell Biol       Date:  1995-06       Impact factor: 10.539

6.  Virulence factors of influenza A viruses: WSN virus neuraminidase required for plaque production in MDBK cells.

Authors:  J L Schulman; P Palese
Journal:  J Virol       Date:  1977-10       Impact factor: 5.103

7.  Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope.

Authors:  D Görlich; S Kostka; R Kraft; C Dingwall; R A Laskey; E Hartmann; S Prehn
Journal:  Curr Biol       Date:  1995-04-01       Impact factor: 10.834

8.  Identification of an RNA binding region within the N-terminal third of the influenza A virus nucleoprotein.

Authors:  C Albo; A Valencia; A Portela
Journal:  J Virol       Date:  1995-06       Impact factor: 5.103

9.  Nucleo-cytoplasmic distribution of human hnRNP proteins: a search for the targeting domains in hnRNP A1.

Authors:  F Weighardt; G Biamonti; S Riva
Journal:  J Cell Sci       Date:  1995-02       Impact factor: 5.285

10.  A nuclear localization domain in the hnRNP A1 protein.

Authors:  H Siomi; G Dreyfuss
Journal:  J Cell Biol       Date:  1995-05       Impact factor: 10.539
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  105 in total

1.  Identification of amino acid residues of influenza virus nucleoprotein essential for RNA binding.

Authors:  D Elton; L Medcalf; K Bishop; D Harrison; P Digard
Journal:  J Virol       Date:  1999-09       Impact factor: 5.103

2.  A beta-stranded motif drives capsid protein oligomers of the parvovirus minute virus of mice into the nucleus for viral assembly.

Authors:  E Lombardo; J C Ramírez; M Agbandje-McKenna; J M Almendral
Journal:  J Virol       Date:  2000-04       Impact factor: 5.103

3.  A nonconventional nuclear localization signal within the UL84 protein of human cytomegalovirus mediates nuclear import via the importin alpha/beta pathway.

Authors:  Peter Lischka; Gabriele Sorg; Michael Kann; Michael Winkler; Thomas Stamminger
Journal:  J Virol       Date:  2003-03       Impact factor: 5.103

Review 4.  Uncovering the global host cell requirements for influenza virus replication via RNAi screening.

Authors:  Silke Stertz; Megan L Shaw
Journal:  Microbes Infect       Date:  2011-01-27       Impact factor: 2.700

5.  Nuclear MxA proteins form a complex with influenza virus NP and inhibit the transcription of the engineered influenza virus genome.

Authors:  Kadir Turan; Masaki Mibayashi; Kenji Sugiyama; Shoko Saito; Akiko Numajiri; Kyosuke Nagata
Journal:  Nucleic Acids Res       Date:  2004-01-29       Impact factor: 16.971

6.  Identification and characterization of three novel nuclear export signals in the influenza A virus nucleoprotein.

Authors:  Maorong Yu; Xiaoling Liu; Shuai Cao; Zhendong Zhao; Ke Zhang; Qing Xie; Caiwei Chen; Shengyan Gao; Yuhai Bi; Lei Sun; Xin Ye; George F Gao; Wenjun Liu
Journal:  J Virol       Date:  2012-02-15       Impact factor: 5.103

7.  Generation and comprehensive analysis of an influenza virus polymerase cellular interaction network.

Authors:  Lionel Tafforeau; Thibault Chantier; Fabrine Pradezynski; Johann Pellet; Philippe E Mangeot; Pierre-Olivier Vidalain; Patrice Andre; Chantal Rabourdin-Combe; Vincent Lotteau
Journal:  J Virol       Date:  2011-10-12       Impact factor: 5.103

8.  Cellular splicing factor RAF-2p48/NPI-5/BAT1/UAP56 interacts with the influenza virus nucleoprotein and enhances viral RNA synthesis.

Authors:  F Momose; C F Basler; R E O'Neill; A Iwamatsu; P Palese; K Nagata
Journal:  J Virol       Date:  2001-02       Impact factor: 5.103

9.  Involvement of Hsp90 in assembly and nuclear import of influenza virus RNA polymerase subunits.

Authors:  Tadasuke Naito; Fumitaka Momose; Atsushi Kawaguchi; Kyosuke Nagata
Journal:  J Virol       Date:  2006-11-22       Impact factor: 5.103

10.  Contributions of two nuclear localization signals of influenza A virus nucleoprotein to viral replication.

Authors:  Makoto Ozawa; Ken Fujii; Yukiko Muramoto; Shinya Yamada; Seiya Yamayoshi; Ayato Takada; Hideo Goto; Taisuke Horimoto; Yoshihiro Kawaoka
Journal:  J Virol       Date:  2006-10-18       Impact factor: 5.103
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