Computational neural networks for predictive microbiology. II. Application to microbial growth.

The growth of a specific microorganism on a certain food is influenced by a number of environmental factors such as temperature, pH, and salt concentration. Methods that delineate the history of the growth of microorganisms are always subject to a considerable debate and scrutiny in the field of predictive microbiology. Regardless of its types, a growth model (e.g., modified Gompertz model) contains several parameters that vary depending on the microorganisms/food combination and the associated prevailing environmental conditions. The growth model parameters for a set of operating conditions are commonly determined from expressions developed via multiple linear regressions. In the present study, a substitute for the nonlinear regression-based equations is developed using computational neural networks. Computational neural networks are applied herein on experimental data pertaining to the anaerobic growth of Shigella flexneri. Results have indicated that predictions by neural networks offer better agreement with experimental data as compared to predictions obtained via corresponding regression equations.