Literature DB >> 9028054

High efficiency transformation of Bacillus brevis by electroporation.

A Okamoto1, A Kosugi, Y Koizumi, F Yanagida, S Udaka.   

Abstract

Various conditions in the Bacillus brevis transformation by electroporation were investigated to increase the efficiency. Competent cell volume and presence of polyethylene glycol 6000 and single stranded DNA on pulsing were critical for the efficiency. As a result, we established the improved method by which 10(7)-10(8) transformants/microgram plasmid DNA could be obtained.

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Year:  1997        PMID： 9028054     DOI： 10.1271/bbb.61.202

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  6 in total

1.  A protein disulfide isomerase gene fusion expression system that increases the extracellular productivity of Bacillus brevis.

Authors:  T Kajino; C Ohto; M Muramatsu; S Obata; S Udaka; Y Yamada; H Takahashi
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

2.  Efficient production of artificially designed gelatins with a Bacillus brevis system.

Authors:  T Kajino; H Takahashi; M Hirai; Y Yamada
Journal:  Appl Environ Microbiol       Date:  2000-01       Impact factor: 4.792

3.  An Improved Method of Preparing High Efficiency Transformation Escherichia coli with Both Plasmids and Larger DNA Fragments.

Authors:  Jingjing Liu; Wenwen Chang; Lei Pan; Xiaoyun Liu; Lufang Su; Weiying Zhang; Qin Li; Yu Zheng
Journal:  Indian J Microbiol       Date:  2018-05-29       Impact factor: 2.461

4.  Recombineering and MAGE.

Authors:  Timothy M Wannier; Peter N Ciaccia; Andrew D Ellington; Gabriel T Filsinger; Farren J Isaacs; Kamyab Javanmardi; Michaela A Jones; Aditya M Kunjapur; Akos Nyerges; Csaba Pal; Max G Schubert; George M Church
Journal:  Nat Rev Methods Primers       Date:  2021-01-14

5.  Improving the insecticidal activity against resistant Culex quinquefasciatus mosquitoes by expression of chitinase gene chiAC in Bacillus sphaericus.

Authors:  Yajun Cai; Jianpin Yan; Xiaomin Hu; Bei Han; Zhiming Yuan
Journal:  Appl Environ Microbiol       Date:  2007-10-12       Impact factor: 4.792

6.  Enhancement of edeine production in Brevibacillus brevis X23 via in situ promoter engineering.

Authors:  Qingshu Liu; Liang Zhang; Yunsheng Wang; Cuiyang Zhang; Tianbo Liu; Caichen Duan; Xiaoying Bian; Zhaohui Guo; Qingshan Long; Ying Tang; Jie Du; Aiyu Liu; Liangying Dai; Dingjun Li; Wu Chen
Journal:  Microb Biotechnol       Date:  2021-07-26       Impact factor: 5.813
  6 in total

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