Literature DB >> 9028006

Cloning and characterization of cDNAs encoding three isoforms of phospholipase A2 in Malayan spitting cobra (Naja naja sputatrix) venom.

A Armugam1, L Earnest, M C Chung, P Gopalakrishnakone, C H Tan, N H Tan, K Jeyaseelan.   

Abstract

cDNAs encoding three phospholipase A2 (PLA2) isoforms in Naja naja sputatrix were cloned and characterized. One of them encoded an acidic PLA2 (APLA) while the others encoded neutral PLA2 (NPLA-1 and NPLA-2). The specific characteristics of APLA and NPLA were attributed to mutations at nt139 and nt328 from G to C and G to A, respectively, resulting in amino acid substitutions from Asp20 and 83 in APLA to His20 and Asn83 in NPLA. Amino acid sequencing of purified protein also showed the presence of this Asp20 and His20 in APLA and NPLA, respectively. The cDNA encoding one of the PLA2 (NAJPLA-2A), when expressed in Escherichia coli, yielded a protein that exhibited PLA2 activity.

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Year:  1997        PMID： 9028006     DOI： 10.1016/s0041-0101(96)00071-2

Source DB:  PubMed          Journal:  Toxicon        ISSN： 0041-0101            Impact factor:   3.033


  6 in total

1.  Sputa nerve growth factor forms a preferable substitute to mouse 7S-beta nerve growth factor.

Authors:  Dawn C-I Koh; A Armugam; K Jeyaseelan
Journal:  Biochem J       Date:  2004-10-01       Impact factor: 3.857

2.  Postsynaptic alpha-neurotoxin gene of the spitting cobra, Naja naja sputatrix: structure, organization, and phylogenetic analysis.

Authors:  F Afifiyan; A Armugam; C H Tan; P Gopalakrishnakone; K Jeyaseelan
Journal:  Genome Res       Date:  1999-03       Impact factor: 9.043

3.  Exploring the Diversity and Novelty of Toxin Genes in Naja sumatrana, the Equatorial Spitting Cobra from Malaysia through De Novo Venom-Gland Transcriptomics.

Authors:  Ho Phin Chong; Kae Yi Tan; Nget Hong Tan; Choo Hock Tan
Journal:  Toxins (Basel)       Date:  2019-02-11       Impact factor: 4.546

4.  A secretory phospholipase A2-mediated neuroprotection and anti-apoptosis.

Authors:  Arunmozhiarasi Armugam; Charmian D N Cher; KaiYing Lim; Dawn C I Koh; David W Howells; Kandiah Jeyaseelan
Journal:  BMC Neurosci       Date:  2009-09-23       Impact factor: 3.288

5.  Humanized-single domain antibodies (VH/VHH) that bound specifically to Naja kaouthia phospholipase A2 and neutralized the enzymatic activity.

Authors:  Charnwit Chavanayarn; Jeeraphong Thanongsaksrikul; Kanyarat Thueng-In; Kunan Bangphoomi; Nitat Sookrung; Wanpen Chaicumpa
Journal:  Toxins (Basel)       Date:  2012-07-19       Impact factor: 4.546

6.  Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A2 from Malaysian Spitting Cobra (Naja sumatrana) Venom.

Authors:  Nur Atiqah Haizum Abdullah; Muhamad Rusdi Ahmad Rusmili; Syafiq Asnawi Zainal Abidin; Mohd Farooq Shaikh; Wayne C Hodgson; Iekhsan Othman
Journal:  Toxins (Basel)       Date:  2021-12-02       Impact factor: 4.546
  6 in total

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