Literature DB >> 9026452

Electrotransformation of lactobacillus acidophilus group A1.

D C Walker1, K Aoyama, T R Klaenhammer.   

Abstract

Two strains of Lactobacillus acidophilus Group A1, the neotype ATCC 4356 and a human isolate NCFM-N2, widely used as a dietary adjunct in milk and cultured dairy products, were transformed with plasmid DNA by electroporation. The transformation characteristics exhibited by the two L acidophilus strains were found to differ markedly even though they appeared similar at the genomic level based on the DNA patterns of SmaI restriction fragments. To our knowledge, this is the first report of a consistent, reproducible transformation system of Lactobacillus acidophilus strains comprising the A1 DNA homology group.

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Year:  1996        PMID： 9026452     DOI： 10.1111/j.1574-6968.1996.tb08163.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  27 in total

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Journal:  J Bacteriol       Date:  2007-04-20       Impact factor: 3.490

3.  Microarray analysis of a two-component regulatory system involved in acid resistance and proteolytic activity in Lactobacillus acidophilus.

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4.  An origin of transfer (oriT) on the conjugative element pRS01 from Lactococcus lactis subsp. lactis ML3.

Authors:  D A Mills; T G Phister; G M Dunny; L L McKay
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5.  Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM.

Authors:  B Logan Buck; Eric Altermann; Tina Svingerud; Todd R Klaenhammer
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6.  Directed chromosomal integration and expression of the reporter gene gusA3 in Lactobacillus acidophilus NCFM.

Authors:  Grace L Douglas; Todd R Klaenhammer
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7.  Selecting lactic acid bacteria for their safety and functionality by use of a mouse colitis model.

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8.  Expression of a heterologous manganese superoxide dismutase gene in intestinal lactobacilli provides protection against hydrogen peroxide toxicity.

Authors:  Jose M Bruno-Bárcena; Jason M Andrus; Stephen L Libby; Todd R Klaenhammer; Hosni M Hassan
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9.  Chromosomal insertions in the Lactobacillus casei upp gene that are useful for vaccine expression.

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Journal:  Appl Environ Microbiol       Date:  2014-03-21       Impact factor: 4.792

10.  Transcriptional and functional analysis of oxalyl-coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes from Lactobacillus acidophilus.

Authors:  M Andrea Azcarate-Peril; Jose M Bruno-Bárcena; Hosni M Hassan; Todd R Klaenhammer
Journal:  Appl Environ Microbiol       Date:  2006-03       Impact factor: 4.792
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