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Literature DB >> 9025322

Cloning and expression of pyranose oxidase cDNA from Coriolus versicolor in Escherichia coli.

Abstract

Complementary DNA encoding pyranose oxidase (PROD) was cloned and sequenced for the first time from Coriolus versicolor. The nucleotide sequence revealed an open reading frame encoding a polypeptide composed of 623 amino acid residues. Compared with the experimentally determined N-terminal sequence of the PROD from C. versicolor. 38 amino acids from the N-terminus of the protein appeared to be eliminated during protein maturation. The cDNA was successfully expressed under the control of lacUV5 promoter in Escherichia coli at 25 degrees C, which will be beneficial in industrial production.

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Year: 1996 PMID： 9025322 DOI： 10.1016/s0168-1656(96)01618-5

Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307

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Cited

7 in total

1. Purification and characterization of pyranose oxidase from the white rot fungus Trametes multicolor.

Authors: C Leitner; J Volc; D Haltrich
Journal: Appl Environ Microbiol Date: 2001-08 Impact factor: 4.792

2. Isolation and purification of pyranose 2-oxidase from Phanerochaete chrysosporium and characterization of gene structure and regulation.

Authors: Theodorus H de Koker; Michael D Mozuch; Daniel Cullen; Jill Gaskell; Philip J Kersten
Journal: Appl Environ Microbiol Date: 2004-10 Impact factor: 4.792

3. Membrane-associated glucose-methanol-choline oxidoreductase family enzymes PhcC and PhcD are essential for enantioselective catabolism of dehydrodiconiferyl alcohol.

Authors: Kenji Takahashi; Yusaku Hirose; Naofumi Kamimura; Shojiro Hishiyama; Hirofumi Hara; Takuma Araki; Daisuke Kasai; Shinya Kajita; Yoshihiro Katayama; Masao Fukuda; Eiji Masai
Journal: Appl Environ Microbiol Date: 2015-09-11 Impact factor: 4.792

7. Crystal structures of Phanerochaete chrysosporium pyranose 2-oxidase suggest that the N-terminus acts as a propeptide that assists in homotetramer assembly.

Authors: Noor Hassan; Tien-Chye Tan; Oliver Spadiut; Ines Pisanelli; Laura Fusco; Dietmar Haltrich; Clemens K Peterbauer; Christina Divne
Journal: FEBS Open Bio Date: 2013-11-05 Impact factor: 2.693

7 in total

Cloning and expression of pyranose oxidase cDNA from Coriolus versicolor in Escherichia coli.

1. Purification and characterization of pyranose oxidase from the white rot fungus Trametes multicolor.

2. Isolation and purification of pyranose 2-oxidase from Phanerochaete chrysosporium and characterization of gene structure and regulation.

3. Membrane-associated glucose-methanol-choline oxidoreductase family enzymes PhcC and PhcD are essential for enantioselective catabolism of dehydrodiconiferyl alcohol.

4. Thermostabilization of bacterial fructosyl-amino acid oxidase by directed evolution.

5. Sequencing and characterization of the xyl operon of a gram-positive bacterium, Tetragenococcus halophila.

6. Biochemical and mutational analyses of a Trametes pyranose oxidase and comparison of its mutants in breadmaking.

7. Crystal structures of Phanerochaete chrysosporium pyranose 2-oxidase suggest that the N-terminus acts as a propeptide that assists in homotetramer assembly.