Literature DB >> 9023231

Role of His243 in the phosphatase activity of EnvZ in Escherichia coli.

K Skarphol1, J Waukau, S A Forst.   

Abstract

EnvZ undergoes autophosphorylation at His243 and subsequently transfers the phosphate group to OmpR. EnvZ also possesses an OmpR-phosphate phosphatase activity. We examined the role of His243 in the phosphatase function by replacing His with either Val, Tyr, Ser, Asp, or Asn. EnvZH243V and EnvZH243Y were both shown to possess phosphatase activity in vitro. In addition, the mutant proteins were able to reduce the high level of OmpR-phosphate present in the envZ473 strain. These results indicate that His243 of EnvZ is not essential for stimulating the dephosphorylation of OmpR-phosphate.

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Year:  1997        PMID： 9023231      PMCID： PMC178845          DOI： 10.1128/jb.179.4.1413-1416.1997

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  22 in total

1.  Intermolecular complementation between two defective mutant signal-transducing receptors of Escherichia coli.

Authors:  Y Yang; M Inouye
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-15       Impact factor: 11.205

2.  EnvZ controls the concentration of phosphorylated OmpR to mediate osmoregulation of the porin genes.

Authors:  F D Russo; T J Silhavy
Journal:  J Mol Biol       Date:  1991-12-05       Impact factor: 5.469

3.  cis-acting ompF mutations that result in OmpR-dependent constitutive expression.

Authors:  J M Slauch; T J Silhavy
Journal:  J Bacteriol       Date:  1991-07       Impact factor: 3.490

Review 4.  Signal transduction and gene regulation through the phosphorylation of two regulatory components: the molecular basis for the osmotic regulation of the porin genes.

Authors:  T Mizuno; S Mizushima
Journal:  Mol Microbiol       Date:  1990-07       Impact factor: 3.501

Review 5.  Signal transduction in bacteria: kinases that control gene expression.

Authors:  M M Igo; J M Slauch; T J Silhavy
Journal:  New Biol       Date:  1990-01

6.  In vivo phosphorylation of OmpR, the transcription activator of the ompF and ompC genes in Escherichia coli.

Authors:  S Forst; J Delgado; A Rampersaud; M Inouye
Journal:  J Bacteriol       Date:  1990-06       Impact factor: 3.490

7.  Phosphorylation and dephosphorylation of a bacterial transcriptional activator by a transmembrane receptor.

Authors:  M M Igo; A J Ninfa; J B Stock; T J Silhavy
Journal:  Genes Dev       Date:  1989-11       Impact factor: 11.361

8.  Phosphorylation of nitrogen regulator I (NRI) of Escherichia coli.

Authors:  V Weiss; B Magasanik
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

9.  Phosphorylation of OmpR by the osmosensor EnvZ modulates expression of the ompF and ompC genes in Escherichia coli.

Authors:  S Forst; J Delgado; M Inouye
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

10.  Reverse phosphotransfer from OmpR to EnvZ in a kinase-/phosphatase+ mutant of EnvZ (EnvZ.N347D), a bifunctional signal transducer of Escherichia coli.

Authors:  R Dutta; M Inouye
Journal:  J Biol Chem       Date:  1996-01-19       Impact factor: 5.157
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  20 in total

1.  Novel role for an HPt domain in stabilizing the phosphorylated state of a response regulator domain.

Authors:  F Janiak-Spens; D P Sparling; A H West
Journal:  J Bacteriol       Date:  2000-12       Impact factor: 3.490

2.  The inner membrane histidine kinase EnvZ senses osmolality via helix-coil transitions in the cytoplasm.

Authors:  Loo Chien Wang; Leslie K Morgan; Pahan Godakumbura; Linda J Kenney; Ganesh S Anand
Journal:  EMBO J       Date:  2012-04-27       Impact factor: 11.598

3.  The phosphoryl transfer domain of UhpB interacts with the response regulator UhpA.

Authors:  J S Wright; R J Kadner
Journal:  J Bacteriol       Date:  2001-05       Impact factor: 3.490

4.  Kinetic characterization of the WalRKSpn (VicRK) two-component system of Streptococcus pneumoniae: dependence of WalKSpn (VicK) phosphatase activity on its PAS domain.

Authors:  Alina D Gutu; Kyle J Wayne; Lok-To Sham; Malcolm E Winkler
Journal:  J Bacteriol       Date:  2010-02-26       Impact factor: 3.490

5.  Differential expression of the OmpF and OmpC porin proteins in Escherichia coli K-12 depends upon the level of active OmpR.

Authors:  C Y Lan; M M Igo
Journal:  J Bacteriol       Date:  1998-01       Impact factor: 3.490

6.  Function of conserved histidine-243 in phosphatase activity of EnvZ, the sensor for porin osmoregulation in Escherichia coli.

Authors:  W Hsing; T J Silhavy
Journal:  J Bacteriol       Date:  1997-06       Impact factor: 3.490

7.  Transcriptional regulation of the Enterococcus faecium BM4147 vancomycin resistance gene cluster by the VanS-VanR two-component regulatory system in Escherichia coli K-12.

Authors:  A Haldimann; S L Fisher; L L Daniels; C T Walsh; B L Wanner
Journal:  J Bacteriol       Date:  1997-09       Impact factor: 3.490

Review 8.  How important is the phosphatase activity of sensor kinases?

Authors:  Linda J Kenney
Journal:  Curr Opin Microbiol       Date:  2010-03-10       Impact factor: 7.934

9.  The ompB operon partially determines differential expression of OmpC in Salmonella typhi and Escherichia coli.

Authors:  I Martínez-Flores; R Cano; V H Bustamante; E Calva; J L Puente
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

10.  Cysteine-scanning analysis of the dimerization domain of EnvZ, an osmosensing histidine kinase.

Authors:  Ling Qin; Shengjian Cai; Yan Zhu; Masayori Inouye
Journal:  J Bacteriol       Date:  2003-06       Impact factor: 3.490
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