Literature DB >> 9023229

Identification of the epitope for a highly cross-reactive monoclonal antibody on the major sigma factor of bacterial RNA polymerase.

M J Breyer1, N E Thompson, R R Burgess.   

Abstract

A highly cross-reactive monoclonal antibody (MAb), 2G10, was found to react in a conserved region of Escherichia coli RNA polymerase sigma70. The epitope was localized to amino acids 470 to 486, which included part of conserved region 3.1. The epitope for MAb 3D3, a MAb which maps close to the 2G10 epitope, was also determined.

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Year:  1997        PMID： 9023229      PMCID： PMC178843          DOI： 10.1128/jb.179.4.1404-1408.1997

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  28 in total

1.  Use of in vitro protein synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies.

Authors:  S A Lesley; M A Brow; R R Burgess
Journal:  J Biol Chem       Date:  1991-02-05       Impact factor: 5.157

2.  Use of T7 RNA polymerase to direct expression of cloned genes.

Authors:  F W Studier; A H Rosenberg; J J Dunn; J W Dubendorff
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

3.  Epitope mapping using histidine-tagged protein fragments: application to Escherichia coli RNA polymerase sigma 70.

Authors:  L Rao; D P Jones; L H Nguyen; S A McMahan; R R Burgess
Journal:  Anal Biochem       Date:  1996-10-15       Impact factor: 3.365

4.  Structure and function of the sigma-70 subunit of Escherichia coli RNA polymerase. Monoclonal antibodies: localization of epitopes by peptide mapping and effects on transcription.

Authors:  M S Strickland; N E Thompson; R R Burgess
Journal:  Biochemistry       Date:  1988-07-26       Impact factor: 3.162

5.  Characterization of the Escherichia coli transcription factor sigma 70: localization of a region involved in the interaction with core RNA polymerase.

Authors:  S A Lesley; R R Burgess
Journal:  Biochemistry       Date:  1989-09-19       Impact factor: 3.162

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Identification of subunits of gonococcal RNA polymerase by immunoblot analysis: evidence for multiple sigma factors.

Authors:  K W Klimpel; S A Lesley; V L Clark
Journal:  J Bacteriol       Date:  1989-07       Impact factor: 3.490

8.  Chlamydia trachomatis RNA polymerase major sigma subunit. Sequence and structural comparison of conserved and unique regions with Escherichia coli sigma 70 and Bacillus subtilis sigma 43.

Authors:  J E Koehler; R R Burgess; N E Thompson; R S Stephens
Journal:  J Biol Chem       Date:  1990-08-05       Impact factor: 5.157

9.  Studies of the role of the Escherichia coli heat shock regulatory protein sigma 32 by the use of monoclonal antibodies.

Authors:  S A Lesley; N E Thompson; R R Burgess
Journal:  J Biol Chem       Date:  1987-04-15       Impact factor: 5.157

10.  In vitro use of monoclonal antibodies in Escherichia coli S-30 extracts to determine the RNA polymerase sigma subunit required by a promoter.

Authors:  S B Jovanovich; S A Lesley; R R Burgess
Journal:  J Biol Chem       Date:  1989-03-05       Impact factor: 5.157
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  5 in total

1.  A bacterial ATP-dependent, enhancer binding protein that activates the housekeeping RNA polymerase.

Authors:  W C Bowman; R G Kranz
Journal:  Genes Dev       Date:  1998-06-15       Impact factor: 11.361

2.  Transient association of an alternative sigma factor, ComX, with RNA polymerase during the period of competence for genetic transformation in Streptococcus pneumoniae.

Authors:  Ping Luo; Donald A Morrison
Journal:  J Bacteriol       Date:  2003-01       Impact factor: 3.490

3.  Mycobacterial SigA and SigB Cotranscribe Essential Housekeeping Genes during Exponential Growth.

Authors:  Kelley Hurst-Hess; Rajesh Biswas; Yong Yang; Paulami Rudra; Erica Lasek-Nesselquist; Pallavi Ghosh
Journal:  mBio       Date:  2019-05-21       Impact factor: 7.867

4.  Region 4 of Rhizobium etli Primary Sigma Factor (SigA) Confers Transcriptional Laxity in Escherichia coli.

Authors:  Orlando Santillán; Miguel A Ramírez-Romero; Luis Lozano; Alberto Checa; Sergio M Encarnación; Guillermo Dávila
Journal:  Front Microbiol       Date:  2016-07-13       Impact factor: 5.640

5.  Implantation of Bacillus pseudomycoides Chromate Transporter Increases Chromate Tolerance in Bacillus subtilis.

Authors:  Zuzana Chromiková; Romana Kalianková Chovanová; Dragana Tamindžija; Barbora Bártová; Dragan Radnović; Rizlan Bernier-Latmani; Imrich Barák
Journal:  Front Microbiol       Date:  2022-03-07       Impact factor: 5.640
  5 in total

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