OBJECTIVE: To review application of laser scanning cytometry (LSC) to analyze several different parameters of human solid tumors in relation to the cell cycle. STUDY DESIGN: Tissue sections of cytology specimens were stained for specific parameters and analyzed by LSC. RESULTS: Examples of LSC analysis of the expression of ER, Ki-67, cyclin B1, BrdUrd and ploidy are given. CONCLUSION: LSC provides rapid, high-precision measurement of the chosen parameters or constituents of each cell in a population of cells and correlates those measurements with the visual image of the corresponding cell.
OBJECTIVE: To review application of laser scanning cytometry (LSC) to analyze several different parameters of humansolid tumors in relation to the cell cycle. STUDY DESIGN: Tissue sections of cytology specimens were stained for specific parameters and analyzed by LSC. RESULTS: Examples of LSC analysis of the expression of ER, Ki-67, cyclin B1, BrdUrd and ploidy are given. CONCLUSION: LSC provides rapid, high-precision measurement of the chosen parameters or constituents of each cell in a population of cells and correlates those measurements with the visual image of the corresponding cell.
Authors: J P Gardner; I Frolov; S Perri; Y Ji; M L MacKichan; J zur Megede; M Chen; B A Belli; D A Driver; S Sherrill; C E Greer; G R Otten; S W Barnett; M A Liu; T W Dubensky; J M Polo Journal: J Virol Date: 2000-12 Impact factor: 5.103
Authors: M J Grace; L Xie; M L Musco; S Cui; M Gurnani; R DiGiacomo; A Chang; S Indelicato; J Syed; R Johnson; L L Nielsen Journal: Am J Pathol Date: 1999-12 Impact factor: 4.307
Authors: Hyuk-Sang Kwon; Yoon Sung Nam; Dominika M Wiktor-Brown; Bevin P Engelward; Peter T C So Journal: J R Soc Interface Date: 2009-02-06 Impact factor: 4.118