Literature DB >> 902106

Purification of viable ciliated cuboidal ependymal cells from rat brain.

C M Manthorpe, G P Wilkin, J E Wilson.   

Abstract

Trypsinization of coronal sections of rat brain, followed by incubation with the chelating agent, ethyleneglycol-bis(beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA), results in selective release of ciliated cuboidal ependymal cells which may be further purified by centrifugal methods. The isolated cells are motile, viable, and exhibit ultrastructure comparable to that in situ.

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Year:  1977        PMID： 902106     DOI： 10.1016/0006-8993(77)90818-6

Source DB:  PubMed          Journal:  Brain Res        ISSN： 0006-8993            Impact factor:   3.252


  9 in total

1.  A Novel Monoclonal Antibody Against Neuroepithelial and Ependymal Cells and Characteristics of Its Positive Cells in Neurospheres.

Authors:  Masaharu Kotani; Yasunori Sato; Akemichi Ueno; Toshinori Ito; Kouichi Itoh; Masato Imada
Journal:  Cell Mol Neurobiol       Date:  2015-05-27       Impact factor: 5.046

2.  A simple method to obtain pure cultures of multiciliated ependymal cells from adult rodents.

Authors:  J M Grondona; P Granados-Durán; P Fernández-Llebrez; M D López-Ávalos
Journal:  Histochem Cell Biol       Date:  2012-08-10       Impact factor: 4.304

3.  Immunohistochemical demonstration of glycogen phosphorylase in rat brain slices.

Authors:  B Pfeiffer; K Elmer; W Roggendorf; P H Reinhart; B Hamprecht
Journal:  Histochemistry       Date:  1990

4.  Identification of a hemagglutinin-specific idiotype associated with reovirus recognition shared by lymphoid and neural cells.

Authors:  J T Nepom; H L Weiner; M A Dichter; M Tardieu; D R Spriggs; C F Gramm; M L Powers; B N Fields; M I Greene
Journal:  J Exp Med       Date:  1982-01-01       Impact factor: 14.307

5.  Atrial natriuretic peptides elevate cyclic GMP levels in primary cultures of rat ependymal cells.

Authors:  John Wellard; Mirna Rapp; Bernd Hamprecht; Stephan Verleysdonk
Journal:  Neurochem Res       Date:  2003-02       Impact factor: 3.996

6.  Survey of virally mediated permeability changes.

Authors:  K A Foster; K Gill; K J Micklem; C A Pasternak
Journal:  Biochem J       Date:  1980-09-15       Impact factor: 3.857

7.  Uptake and metabolism of serotonin by ependymal primary cultures.

Authors:  Stephan Verleysdonk; Bernd Hamprecht; Mirna Rapp; John Wellard
Journal:  Neurochem Res       Date:  2004-09       Impact factor: 3.996

8.  Regulation by insulin and insulin-like growth factor of 2-deoxyglucose uptake in primary ependymal cell cultures.

Authors:  Stephan Verleysdonk; Wolfgang Hirschner; John Wellard; Mirna Rapp; Maria de los Angeles Garcia; Francisco Nualart; Bernd Hamprecht
Journal:  Neurochem Res       Date:  2004-01       Impact factor: 3.996

Review 9.  Interaction of viruses with cell surface receptors.

Authors:  M Tardieu; R L Epstein; H L Weiner
Journal:  Int Rev Cytol       Date:  1982
  9 in total

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