Literature DB >> 9016579

Recombination-mediated PCR-directed plasmid construction in vivo in yeast.

K R Oldenburg1, K T Vo, S Michaelis, C Paddon.   

Abstract

We have extended the technique of PCR-directed recombination in Saccharomyces cerevisiae to develop a simple method for plasmid or gene construction in the absence of suitable restriction sites. The DNA to be cloned is PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid. Co-transformation into yeast results in homologous recombination at a position directed by the PCR oligonucleotides.

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Year:  1997        PMID： 9016579      PMCID： PMC146432          DOI： 10.1093/nar/25.2.451

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.

Authors:  R Rothstein
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

2.  Plasmid construction by homologous recombination in yeast.

Authors:  H Ma; S Kunes; P J Schatz; D Botstein
Journal:  Gene       Date:  1987       Impact factor: 3.688

3.  Improved green fluorescent protein by molecular evolution using DNA shuffling.

Authors:  A Crameri; E A Whitehorn; E Tate; W P Stemmer
Journal:  Nat Biotechnol       Date:  1996-03       Impact factor: 54.908

4.  A rapid and reliable DNA preparation method for screening a large number of yeast clones by polymerase chain reaction.

Authors:  M Ling; F Merante; B H Robinson
Journal:  Nucleic Acids Res       Date:  1995-12-11       Impact factor: 16.971

5.  Micro-homology mediated PCR targeting in Saccharomyces cerevisiae.

Authors:  P Manivasakam; S C Weber; J McElver; R H Schiestl
Journal:  Nucleic Acids Res       Date:  1995-07-25       Impact factor: 16.971

6.  A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.

Authors:  A Baudin; O Ozier-Kalogeropoulos; A Denouel; F Lacroute; C Cullin
Journal:  Nucleic Acids Res       Date:  1993-07-11       Impact factor: 16.971

7.  Analysis of the localization of STE6/CFTR chimeras in a Saccharomyces cerevisiae model for the cystic fibrosis defect CFTR delta F508.

Authors:  C Paddon; D Loayza; L Vangelista; R Solari; S Michaelis
Journal:  Mol Microbiol       Date:  1996-03       Impact factor: 3.501

8.  Yeast transformation: a model system for the study of recombination.

Authors:  T L Orr-Weaver; J W Szostak; R J Rothstein
Journal:  Proc Natl Acad Sci U S A       Date:  1981-10       Impact factor: 11.205

9.  Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene.

Authors:  M M Zukowski; D F Gaffney; D Speck; M Kauffmann; A Findeli; A Wisecup; J P Lecocq
Journal:  Proc Natl Acad Sci U S A       Date:  1983-02       Impact factor: 11.205
  9 in total
  237 in total

1.  Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane.

Authors:  O Kerscher; N B Sepuri; R E Jensen
Journal:  Mol Biol Cell       Date:  2000-01       Impact factor: 4.138

2.  Defining the minimal length of sequence homology required for selective gene isolation by TAR cloning.

Authors:  V N Noskov; M Koriabine; G Solomon; M Randolph; J C Barrett; S H Leem; L Stubbs; N Kouprina; V Larionov
Journal:  Nucleic Acids Res       Date:  2001-03-15       Impact factor: 16.971

3.  Genome-scale cloning and expression of individual open reading frames using topoisomerase I-mediated ligation.

Authors:  J A Heyman; J Cornthwaite; L Foncerrada; J R Gilmore; E Gontang; K J Hartman; C L Hernandez; R Hood; H M Hull; W Y Lee; R Marcil; E J Marsh; K M Mudd; M J Patino; T J Purcell; J J Rowland; M L Sindici; J P Hoeffler
Journal:  Genome Res       Date:  1999-04       Impact factor: 9.043

4.  Intein-mediated assembly of a functional beta-glucuronidase in transgenic plants.

Authors:  Jianjun Yang; George C Fox; Tina V Henry-Smith
Journal:  Proc Natl Acad Sci U S A       Date:  2003-03-10       Impact factor: 11.205

5.  Requirement of the N-terminal extension for vacuolar trafficking and transport activity of yeast Ycf1p, an ATP-binding cassette transporter.

Authors:  Deborah L Mason; Susan Michaelis
Journal:  Mol Biol Cell       Date:  2002-12       Impact factor: 4.138

6.  Slk19p of Saccharomyces cerevisiae regulates anaphase spindle dynamics through two independent mechanisms.

Authors:  Kyle A Havens; Melissa K Gardner; Rebecca J Kamieniecki; Michael E Dresser; Dean S Dawson
Journal:  Genetics       Date:  2010-10-05       Impact factor: 4.562

7.  Tools for fungal proteomics: multifunctional neurospora vectors for gene replacement, protein expression and protein purification.

Authors:  Shinji Honda; Eric U Selker
Journal:  Genetics       Date:  2009-01-26       Impact factor: 4.562

8.  An artificial transport metabolon facilitates improved substrate utilization in yeast.

Authors:  Thomas Thomik; Ilka Wittig; Jun-Yong Choe; Eckhard Boles; Mislav Oreb
Journal:  Nat Chem Biol       Date:  2017-09-04       Impact factor: 15.040

9.  NADPH oxidases regulate septin-mediated cytoskeletal remodeling during plant infection by the rice blast fungus.

Authors:  Lauren S Ryder; Yasin F Dagdas; Thomas A Mentlak; Michael J Kershaw; Christopher R Thornton; Martin Schuster; Jisheng Chen; Zonghua Wang; Nicholas J Talbot
Journal:  Proc Natl Acad Sci U S A       Date:  2013-02-04       Impact factor: 11.205

10.  Evidence for diversifying selection at the pyoverdine locus of Pseudomonas aeruginosa.

Authors:  Eric E Smith; Elizabeth H Sims; David H Spencer; Rajinder Kaul; Maynard V Olson
Journal:  J Bacteriol       Date:  2005-03       Impact factor: 3.490
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