Literature DB >> 9016305

Quadruple immunofluorescence: a direct visualization method.

G L Ferri1, R M Gaudio, I F Castello, P Berger, G Giro.   

Abstract

We describe fluorescence immunostaining of four different antigens in the same section. The fluorochrome conjugates used show highest emission in the blue, green, yellow, and red regions of the visible light spectrum, respectively. Specially designed single fluorochrome filter combinations allow selective visualization of each fluorochrome label in turn, without visible crosstalk with the others.

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Year:  1997        PMID： 9016305     DOI： 10.1177/002215549704500201

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  4 in total

1.  Fully automated 5-plex fluorescent immunohistochemistry with tyramide signal amplification and same species antibodies.

Authors:  Wenjun Zhang; Antony Hubbard; Tobin Jones; Adriana Racolta; Srabani Bhaumik; Nick Cummins; Liping Zhang; Karl Garsha; Frank Ventura; Mark R Lefever; Zhenqiang Lu; John K Hurley; William A Day; Lidija Pestic-Dragovich; Larry E Morrison; Lei Tang
Journal:  Lab Invest       Date:  2017-05-15       Impact factor: 5.662

2.  Multiple antigen immunostaining procedures.

Authors:  Tibor Krenacs; Laszlo Krenacs; Mark Raffeld
Journal:  Methods Mol Biol       Date:  2010

3.  Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue.

Authors:  David Robertson; Kay Savage; Jorge S Reis-Filho; Clare M Isacke
Journal:  BMC Cell Biol       Date:  2008-03-19       Impact factor: 4.241

Review 4.  Garbage in, garbage out: a critical evaluation of strategies used for validation of immunohistochemical biomarkers.

Authors:  Gillian O'Hurley; Evelina Sjöstedt; Arman Rahman; Bo Li; Caroline Kampf; Fredrik Pontén; William M Gallagher; Cecilia Lindskog
Journal:  Mol Oncol       Date:  2014-03-21       Impact factor: 6.603
  4 in total

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