Amplification, cloning and sequencing of the sigmaC-encoded gene of avian reovirus.

The sigmaC-encoding cDNA of avian reovirus (ARV) 1733 strain was amplified, cloned and sequenced using double nested polymerase chain reaction (PCR). The ARV sigmaC protein is a minor component of the outer capsid that induces type-specific neutralization antibodies. Four overlapping sigmaC-encoding cDNA fragments were obtained. Together, the four fragments represented the whole coding sequence. The nucleotide and deduced amino acid sequences of sigmaC-encoded gene of U.S. (S1133 and 1733) and Australian isolates (RAM-1 and SOM-4) were compared. The U.S. isolates were closely related, but different from Australian isolates. The degree of differences between the U.S. and Australian isolates was over 44.89% at both the nucleotide and deduced amino acid levels and suggested that the virus is evolving separately in different continents. The deduced amino acid sequences of ARV sigmaC indicated a heptapeptide repeat in the N-terminal region of ARV sigmaC existed in all ARVs. The results suggested that ARV sigmaC is structurally related to mammalian reovirus (MRV) sigma1.