M M Mahadevan1, M M Miller. 1. Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Abstract
PURPOSE: Our aim was to determine the effect of temperature and incubation time on the toxicity of propanediol on mouse zygotes. METHOD: Zygotes were pooled and randomly allocated to one of the treatment groups. Zygotes were incubated in PBS or PBS containing 1.5M propanediol at 37 degrees C or room temperature (RT) for up to 30 min, washed, and cultured. Similarly, zygotes were incubated at 4 degrees C or RT for 30 min and either washed and cultured or frozen-thawed, washed, and cultured. Zygotes were examined at 24, 48, and 96 hr of culture and embryo quality was determined. RESULTS: Exposure of zygotes to 1.5 M propanediol at 37 degrees C significantly impaired first cleavage and blastocyst formation compared to exposure at RT (P < 0.0007). Incubation of zygotes for more than 5 min at 37 degrees C significantly reduced embryo development (P < 0.00001). Exposure to propanediol at 4 degrees C results in similar embryo development before and after freeze-thawing compared to exposure at RT. CONCLUSIONS: Propanediol is toxic to mouse zygotes in a temperature- and time-dependent fashion. Cryopreservation of zygotes after exposure at 4 degrees C appears to be no better than after exposure at RT.
PURPOSE: Our aim was to determine the effect of temperature and incubation time on the toxicity of propanediol on mouse zygotes. METHOD: Zygotes were pooled and randomly allocated to one of the treatment groups. Zygotes were incubated in PBS or PBS containing 1.5M propanediol at 37 degrees C or room temperature (RT) for up to 30 min, washed, and cultured. Similarly, zygotes were incubated at 4 degrees C or RT for 30 min and either washed and cultured or frozen-thawed, washed, and cultured. Zygotes were examined at 24, 48, and 96 hr of culture and embryo quality was determined. RESULTS: Exposure of zygotes to 1.5 M propanediol at 37 degrees C significantly impaired first cleavage and blastocyst formation compared to exposure at RT (P < 0.0007). Incubation of zygotes for more than 5 min at 37 degrees C significantly reduced embryo development (P < 0.00001). Exposure to propanediol at 4 degrees C results in similar embryo development before and after freeze-thawing compared to exposure at RT. CONCLUSIONS:Propanediol is toxic to mouse zygotes in a temperature- and time-dependent fashion. Cryopreservation of zygotes after exposure at 4 degrees C appears to be no better than after exposure at RT.
Authors: L L Veeck; C H Amundson; L J Brothman; C DeScisciolo; M K Maloney; S J Muasher; H W Jones Journal: Fertil Steril Date: 1993-06 Impact factor: 7.329