Literature DB >> 9008806

Differential reactivity of a monoclonal antibody directed to the membrane protein of porcine reproductive and respiratory syndrome virus.

R Magar1, R Larochelle, E A Nelson, C Charreyre.   

Abstract

A monoclonal antibody (2C12) against the 19 kDa membrane (M) protein of a Canadian isolate of porcine reproductive and respiratory syndrome (PRRS) virus was produced. By indirect immunofluorescence (IIF) cytoplasmic fluorescence was observed in infected cells, but the pattern of fluorescence was generally different and intensity was weaker than that observed using the nucleocapsid protein-directed monoclonal antibody SDOW17. When tested by IIF towards a total of 26 PRRS virus isolates from Canada, 122 isolates from the US and 13 isolates from Europe the 2C12 MAb reacted with all the North American isolates tested including the VR-2332 isolate and the vaccine (RespPRRS) isolate. However no reactivity was observed towards the European isolates tested including the Lelystad virus. This reactivity pattern suggests that the epitope recognized by this MAb on the M protein of PRRS virus appears highly conserved among North American isolates but absent or weakly expressed on European isolates of PRRS virus.

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Year:  1997        PMID： 9008806      PMCID： PMC1189374     

Source DB:  PubMed          Journal:  Can J Vet Res        ISSN： 0830-9000            Impact factor:   1.310


  14 in total

1.  Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332).

Authors:  D A Benfield; E Nelson; J E Collins; L Harris; S M Goyal; D Robison; W T Christianson; R B Morrison; D Gorcyca; D Chladek
Journal:  J Vet Diagn Invest       Date:  1992-04       Impact factor: 1.279

2.  Isolation of a distinct serotype of porcine reproductive and respiratory syndrome (PRRS) virus in Ontario.

Authors:  C A Gagnon; G Milane; S Dea; S Carman; G Charbonneau
Journal:  Can Vet J       Date:  1995-12       Impact factor: 1.008

3.  Porcine reproductive and respiratory syndrome virus identification in proliferative and necrotizing pneumonia cases from Ontario.

Authors:  R Magar; R Larochelle; S Carman; G Thomson
Journal:  Can Vet J       Date:  1994-08       Impact factor: 1.008

4.  Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs.

Authors:  J E Collins; D A Benfield; W T Christianson; L Harris; J C Hennings; D P Shaw; S M Goyal; S McCullough; R B Morrison; H S Joo
Journal:  J Vet Diagn Invest       Date:  1992-04       Impact factor: 1.279

5.  Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies.

Authors:  E A Nelson; J Christopher-Hennings; T Drew; G Wensvoort; J E Collins; D A Benfield
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

6.  Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line.

Authors:  H S Kim; J Kwang; I J Yoon; H S Joo; M L Frey
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

7.  Mystery swine disease in The Netherlands: the isolation of Lelystad virus.

Authors:  G Wensvoort; C Terpstra; J M Pol; E A ter Laak; M Bloemraad; E P de Kluyver; C Kragten; L van Buiten; A den Besten; F Wagenaar
Journal:  Vet Q       Date:  1991-07       Impact factor: 3.320

8.  Characterization of proteins encoded by ORFs 2 to 7 of Lelystad virus.

Authors:  J J Meulenberg; A Petersen-den Besten; E P De Kluyver; R J Moormann; W M Schaaper; G Wensvoort
Journal:  Virology       Date:  1995-01-10       Impact factor: 3.616

9.  Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group.

Authors:  K K Conzelmann; N Visser; P Van Woensel; H J Thiel
Journal:  Virology       Date:  1993-03       Impact factor: 3.616

10.  Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV.

Authors:  J J Meulenberg; M M Hulst; E J de Meijer; P L Moonen; A den Besten; E P de Kluyver; G Wensvoort; R J Moormann
Journal:  Virology       Date:  1993-01       Impact factor: 3.616
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  6 in total

1.  Importance of M-protein C terminus as substrate antigen for serodetection of equine arteritis virus infection.

Authors:  Célia Jeronimo; Denis Archambault
Journal:  Clin Diagn Lab Immunol       Date:  2002-05

2.  Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies.

Authors:  Israrul H Ansari; Byungjoon Kwon; Fernando A Osorio; Asit K Pattnaik
Journal:  J Virol       Date:  2006-04       Impact factor: 5.103

3.  Validation of a blocking enzyme-linked immunosorbent assay for detection of antibodies against porcine reproductive and respiratory syndrome virus.

Authors:  Neal H Ferrin; Ying Fang; Craig R Johnson; Michael P Murtaugh; Dale D Polson; Montserrat Torremorell; Marie L Gramer; Eric A Nelson
Journal:  Clin Diagn Lab Immunol       Date:  2004-05

4.  Characterization of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States.

Authors:  Susan L Ropp; Carrie E Mahlum Wees; Ying Fang; Eric A Nelson; Kurt D Rossow; Melissa Bien; Bill Arndt; Sarah Preszler; Pamela Steen; Jane Christopher-Hennings; James E Collins; David A Benfield; Kay S Faaberg
Journal:  J Virol       Date:  2004-04       Impact factor: 5.103

Review 5.  Heterogeneity of porcine reproductive and respiratory syndrome virus: implications for current vaccine efficacy and future vaccine development.

Authors:  X J Meng
Journal:  Vet Microbiol       Date:  2000-06-12       Impact factor: 3.293

6.  The 2b protein as a minor structural component of PRRSV.

Authors:  Wai-Hong Wu; Ying Fang; Raymond R R Rowland; Steven R Lawson; Jane Christopher-Hennings; Kyoung-Jin Yoon; Eric A Nelson
Journal:  Virus Res       Date:  2005-08-10       Impact factor: 3.303
  6 in total

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