Assay of amylase and isoamylase activities in serum and urine. Modifications in methods and range of normal values.

Certain modifications are described in assay methods previously reported from this laboratory for total amylase and isoamylase activities. These modifications provided more consistent results and achieve superior separation of pancreatic and salivary type (P- and S-type) isoamylases. The estimated range of values in the serum and urine of normal subjects using the modified assay procedures is presented for total amylase as well as for P- and S-type isoamylases.