Synthesis and application of boronic acid-immobilized porous polymer particles: a novel packing for high-performance liquid affinity chromatography.

A preparation method of a novel type of packing materials for high-performance liquid affinity chromatography to determine glycated proteins was studied, and a fundamental study on its application to diabetic serum was conducted. To quantify glycated proteins such as glycated serum albumin, a new hydrophilic and durable porous polymer particle recently developed in our laboratory was used as the basic matrix. The matrix was activated with 1,1'-carbonyldiimidazole (CDI), and optimization of the coupling reaction between these CDI-activated matrix and m-aminophenylboronic acid hemisulfate (APBA) as affinity ligand was investigated. The optimum value for the APBA coupling yield was found to be obtained under acidic conditions very different from the data reported by previous workers. Using this APBA-immobilized matrix an affinity column was prepared, and its usefulness in HPLC separation of glycated serum proteins was investigated. Also, the fundamental and preliminary results for diagnosis of diabetes mellitus are discussed in this paper.