PURPOSE: To define the threshold ethanol concentration that is toxic to cultured cells. METHODS: Three malignant cell lines and freshly isolated normal rat hepatocytes were exposed to 0-50% (vol.) ethanol (concentrations used were 0, 5, 10, 15, 20, 25, 30, 40 and 50%) on tissue culture plates for 0.25-60 min (exposure times used were 0.25, 5, 10, 20, 30, 40 50 and 60 min). Cytotoxicity was estimated by trypan blue exclusion test and from 3H-thymidine incorporation. RESULTS: All cells were killed by a 15-s exposure to 30-40% ethanol while a concentration as low as 15-20% gave a total response after 5-10-min exposures. After a one-hour exposure of F9 carcinoma cells and hepatocytes, a total or nearly total response was achieved with 10% ethanol. The cytotoxic effect was thus dependent both on the exposure time and on the concentration of ethanol. There were no significant differences in ethanol tolerance among the cell types. CONCLUSION: Ethanol seemed to kill cells in the cell culture effectively in much lower concentrations than those currently used in tumour ablation.
PURPOSE: To define the threshold ethanol concentration that is toxic to cultured cells. METHODS: Three malignant cell lines and freshly isolated normal rat hepatocytes were exposed to 0-50% (vol.) ethanol (concentrations used were 0, 5, 10, 15, 20, 25, 30, 40 and 50%) on tissue culture plates for 0.25-60 min (exposure times used were 0.25, 5, 10, 20, 30, 40 50 and 60 min). Cytotoxicity was estimated by trypan blue exclusion test and from 3H-thymidine incorporation. RESULTS: All cells were killed by a 15-s exposure to 30-40% ethanol while a concentration as low as 15-20% gave a total response after 5-10-min exposures. After a one-hour exposure of F9 carcinoma cells and hepatocytes, a total or nearly total response was achieved with 10% ethanol. The cytotoxic effect was thus dependent both on the exposure time and on the concentration of ethanol. There were no significant differences in ethanol tolerance among the cell types. CONCLUSION:Ethanol seemed to kill cells in the cell culture effectively in much lower concentrations than those currently used in tumour ablation.
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Authors: Robert Morhard; Corrine Nief; Carlos Barrero Castedo; Fangyao Hu; Megan Madonna; Jenna L Mueller; Mark W Dewhirst; David F Katz; Nirmala Ramanujam Journal: Sci Rep Date: 2017-08-18 Impact factor: 4.379
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Authors: Enrico M Zardi; Francesco Di Matteo; Daniele Santini; Valentina Uwechie; Pierfilippo Crucitti; Massimiliano Carassiti; Antonio Picardi; Eleonora Perrella; Marco Caricato; Giuseppe Tonini; Roberto Coppola; Antonella Afeltra Journal: J Exp Clin Cancer Res Date: 2008-08-14