Literature DB >> 8992382

Visualization of protein kinase activities in single cells by antibodies against phosphorylated vimentin and GFAP.

N Inagaki1, K Tsujimura, J Tanaka, M Sekimata, Y Kamei, M Inagaki.   

Abstract

Vimentin and glial fibrillary acidic protein (GFAP) are intermediate filament proteins expressed in the cytoplasm of various types of cells. The head domains of these proteins are phosphorylated by various protein kinases. Site- and phosphorylation-specific antibodies which recognize a phosphorylated serine/threonine residue in the head domains and its flanking sequence provide a useful tool to monitor and visualize protein kinase activities in single cells.

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Year:  1996        PMID： 8992382     DOI： 10.1007/bf02532302

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  27 in total

1.  Autophosphorylation of type II Ca2+/calmodulin-dependent protein kinase in cultures of postnatal rat hippocampal slices.

Authors:  S S Molloy; M B Kennedy
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-01       Impact factor: 11.205

2.  Activation of protein kinase C and specific phosphorylation of a Mr 90,000 membrane protein of promotable BALB/3T3 and C3H/10T1/2 cells by tumor promoters.

Authors:  K Chida; H Hashiba; K Sasaki; T Kuroki
Journal:  Cancer Res       Date:  1986-03       Impact factor: 12.701

3.  The multifunctional Ca2+/calmodulin-dependent protein kinase mediates Ca2+-dependent phosphorylation of tyrosine hydroxylase.

Authors:  L C Griffith; H Schulman
Journal:  J Biol Chem       Date:  1988-07-05       Impact factor: 5.157

4.  The growth of research on protein phosphorylation.

Authors:  E G Krebs
Journal:  Trends Biochem Sci       Date:  1994-11       Impact factor: 13.807

5.  Site-specific phosphorylation induces disassembly of vimentin filaments in vitro.

Authors:  M Inagaki; Y Nishi; K Nishizawa; M Matsuyama; C Sato
Journal:  Nature       Date:  1987 Aug 13-19       Impact factor: 49.962

6.  Phorbol esters increase the amount of Ca2+, phospholipid-dependent protein kinase associated with plasma membrane.

Authors:  A S Kraft; W B Anderson
Journal:  Nature       Date:  1983 Feb 17-23       Impact factor: 49.962

7.  Single type-2 astrocytes show multiple independent sites of Ca2+ signaling in response to histamine.

Authors:  N Inagaki; H Fukui; S Ito; A Yamatodani; H Wada
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-15       Impact factor: 11.205

8.  Identification of phosphorylation sites on glial fibrillary acidic protein for cdc2 kinase and Ca(2+)-calmodulin-dependent protein kinase II.

Authors:  K Tsujimura; J Tanaka; S Ando; Y Matsuoka; M Kusubata; H Sugiura; T Yamauchi; M Inagaki
Journal:  J Biochem       Date:  1994-08       Impact factor: 3.387

9.  Visualization and function of vimentin phosphorylation by cdc2 kinase during mitosis.

Authors:  K Tsujimura; M Ogawara; Y Takeuchi; S Imajoh-Ohmi; M H Ha; M Inagaki
Journal:  J Biol Chem       Date:  1994-12-09       Impact factor: 5.157

Review 10.  Glial fibrillary acidic protein: dynamic property and regulation by phosphorylation.

Authors:  M Inagaki; Y Nakamura; M Takeda; T Nishimura; N Inagaki
Journal:  Brain Pathol       Date:  1994-07       Impact factor: 6.508
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  7 in total

1.  Ontogenetic changes in glial fibrillary acid protein phosphorylation, glutamate uptake and glutamine synthetase activity in olfactory bulb of rats.

Authors:  Cíntia Eickhoff Battú; Graça F R S Godinho; Ana Paula Thomazi; Lúcia M V de Almeida; Carlos Alberto Gonçalves; Trícia Kommers; Susana T Wofchuk
Journal:  Neurochem Res       Date:  2005-09       Impact factor: 3.996

Review 2.  Role of the cytoskeleton in formation and maintenance of angiogenic sprouts.

Authors:  Kayla J Bayless; Greg A Johnson
Journal:  J Vasc Res       Date:  2011-04-04       Impact factor: 1.934

3.  Myofibril breakdown during atrophy is a delayed response requiring the transcription factor PAX4 and desmin depolymerization.

Authors:  Alexandra Volodin; Idit Kosti; Alfred Lewis Goldberg; Shenhav Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  2017-01-17       Impact factor: 11.205

4.  Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein.

Authors:  Catherine Su Hui Teo; Justin Jang Hann Chu
Journal:  J Virol       Date:  2013-11-27       Impact factor: 5.103

5.  GSK3-β promotes calpain-1-mediated desmin filament depolymerization and myofibril loss in atrophy.

Authors:  Dina Aweida; Inga Rudesky; Alexandra Volodin; Eitan Shimko; Shenhav Cohen
Journal:  J Cell Biol       Date:  2018-07-30       Impact factor: 10.539

6.  Ubiquitylation by Trim32 causes coupled loss of desmin, Z-bands, and thin filaments in muscle atrophy.

Authors:  Shenhav Cohen; Bo Zhai; Steven P Gygi; Alfred L Goldberg
Journal:  J Cell Biol       Date:  2012-08-20       Impact factor: 10.539

7.  The Serine/threonine kinase Stk33 exhibits autophosphorylation and phosphorylates the intermediate filament protein Vimentin.

Authors:  Bastienne Brauksiepe; Alejandro O Mujica; Harald Herrmann; Erwin R Schmidt
Journal:  BMC Biochem       Date:  2008-09-23       Impact factor: 4.059
  7 in total

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