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Literature DB >> 8992312

Structural analysis of the glycosyl-phosphatidylinositol membrane anchor of the merozoite surface proteins-1 and -2 of Plasmodium falciparum.

P Gerold¹, L Schofield, M J Blackman, A A Holder, R T Schwarz.

Abstract

Plasmodium falciparum accumulates the two merozoite surface proteins-1 and -2 during schizogony. Both proteins are proposed to be anchored in membranes by glycosyl-phosphatidylinositol membrane anchors. In this report the identity of these GPI-anchors is confirmed by labelling with tritiated precursors and additionally by specific enzymatic and chemical treatments. Detailed structural analysis of the core-glycans showed that the GPI-anchors of both proteins possess an extra alpha 1-2 linked mannose at the conserved trimannosyl-core-glycan. MSP-1 and MSP-2 labelled with tritiated myristic acid possess primarily radioactive myristic acid at inositol rings in both GPI-anchors. Additionally the hydrophobic fragments released from [3H]myristic acid labelled GPI-anchors were identified as diacyl-glycerols, carrying preferentially [3H]palmitic acid in an ester-linkage.

Entities: Chemical

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Year: 1996 PMID： 8992312 DOI： 10.1016/0166-6851(95)02518-9

Source DB: PubMed Journal: Mol Biochem Parasitol ISSN： 0166-6851 Impact factor: 1.759

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Cited

60 in total

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Authors: S P Franzot; T L Doering
Journal: Biochem J Date: 1999-05-15 Impact factor: 3.857

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Journal: Eukaryot Cell Date: 2010-05-14

5. Specificity of GlcNAc-PI de-N-acetylase of GPI biosynthesis and synthesis of parasite-specific suicide substrate inhibitors.

Authors: T K Smith; A Crossman; C N Borissow; M J Paterson; A Dix; J S Brimacombe; M A Ferguson
Journal: EMBO J Date: 2001-07-02 Impact factor: 11.598

6. A recombinant vaccine expressed in the milk of transgenic mice protects Aotus monkeys from a lethal challenge with Plasmodium falciparum.

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Journal: Proc Natl Acad Sci U S A Date: 2001-12-18 Impact factor: 11.205

7. Solution conformation, backbone dynamics and lipid interactions of the intrinsically unstructured malaria surface protein MSP2.

Authors: Xuecheng Zhang; Matthew A Perugini; Shenggen Yao; Christopher G Adda; Vincent J Murphy; Andrew Low; Robin F Anders; Raymond S Norton
Journal: J Mol Biol Date: 2008-03-28 Impact factor: 5.469