Literature DB >> 8991645

Immunological characterization of a recombinant 27-kilodalton antigenic protein from Paracoccidioides brasiliensis.

B L Ortiz1, A M Garcia, A Restrepo, J G McEwen.   

Abstract

We report the expression in Escherichia coli of a 27-kDa antigenic protein from Paracoccidioides brasiliensis. When analyzed by immunoblotting, this recombinant antigenic protein was recognized by antibodies present in the sera of 40 of the 44 paracoccidioidomycosis patients studied. No cross-reactions were observed with sera from patients with other mycoses (histoplasmosis, aspergillosis, cryptococcosis, sporotrichosis, and chromoblastomycosis) or with tuberculosis.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8991645      PMCID： PMC170290          DOI： 10.1128/cdli.3.2.239-241.1996

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  12 in total

1.  In vivo excision properties of bacteriophage lambda ZAP expression vectors.

Authors:  J M Short; J A Sorge
Journal:  Methods Enzymol       Date:  1992       Impact factor: 1.600

2.  43-kilodalton glycoprotein from Paracoccidioides brasiliensis: immunochemical reactions with sera from patients with paracoccidioidomycosis, histoplasmosis, or Jorge Lobo's disease.

Authors:  R Puccia; L R Travassos
Journal:  J Clin Microbiol       Date:  1991-08       Impact factor: 5.948

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Exocellular components of Paracoccidioides brasiliensis: identification of a specific antigen.

Authors:  R Puccia; S Schenkman; P A Gorin; L R Travassos
Journal:  Infect Immun       Date:  1986-07       Impact factor: 3.441

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Predictive value of serologic tests in the diagnosis and follow-up of patients with paracoccidioidomycosis.

Authors:  L E Cano; A Restrepo
Journal:  Rev Inst Med Trop Sao Paulo       Date:  1987 Sep-Oct       Impact factor: 1.846

7.  Growth of Paracoccidioides brasiliensis yeast phase in a chemically defined culture medium.

Authors:  A Restrepo; B E Jiménez
Journal:  J Clin Microbiol       Date:  1980-08       Impact factor: 5.948

8.  Characterization of the cellular antigens of Paracoccidioides brasiliensis yeast form.

Authors:  M Casotto
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

9.  Isolation and antigenicity of a 45-kilodalton Paracoccidioides brasiliensis immunodominant antigen.

Authors:  M F Ferreira-da-Cruz; B Galvão-Castro; C Daniel-Ribeiro
Journal:  Infect Immun       Date:  1992-07       Impact factor: 3.441

10.  Diagnosis of paracoccidioidomycosis by dot immunobinding assay for antibody detection using the purified and specific antigen gp43.

Authors:  C P Taborda; Z P Camargo
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948
View more
  7 in total

1.  Combined use of Paracoccidioides brasiliensis recombinant 27-kilodalton and purified 87-kilodalton antigens in an enzyme-linked immunosorbent assay for serodiagnosis of paracoccidioidomycosis.

Authors:  Soraya Díez; Beatriz L Gómez; Juan G McEwen; Angela Restrepo; Rod J Hay; Andrew J Hamilton
Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

2.  Expression of antibodies directed to Paracoccidioides brasiliensis glycosphingolipids during the course of paracoccidioidomycosis treatment.

Authors:  Silvio Bertini; Arnaldo L Colombo; Helio K Takahashi; Anita H Straus
Journal:  Clin Vaccine Immunol       Date:  2006-11-29

3.  Use of the 27-kilodalton recombinant protein from Paracoccidioides brasiliensis in serodiagnosis of paracoccidioidomycosis.

Authors:  B L Ortiz; S Díez; M E Urán; J M Rivas; M Romero; V Caicedo; A Restrepo; J G McEwen
Journal:  Clin Diagn Lab Immunol       Date:  1998-11

Review 4.  Current status of nonculture methods for diagnosis of invasive fungal infections.

Authors:  Siew Fah Yeo; Brian Wong
Journal:  Clin Microbiol Rev       Date:  2002-07       Impact factor: 26.132

5.  Identification and purification of specific Penicillium marneffei antigens and their recognition by human immune sera.

Authors:  L Jeavons; A J Hamilton; N Vanittanakom; R Ungpakorn; E G Evans; T Sirisanthana; R J Hay
Journal:  J Clin Microbiol       Date:  1998-04       Impact factor: 5.948

6.  Additive effect of rPb27 immunization and chemotherapy in experimental paracoccidioidomycosis.

Authors:  Viviane C Fernandes; Estefânia M N Martins; Jankerle N Boeloni; Juliana B Coitinho; Rogéria Serakides; Alfredo M Goes
Journal:  PLoS One       Date:  2011-03-10       Impact factor: 3.240

7.  Evidence for positive selection in putative virulence factors within the Paracoccidioides brasiliensis species complex.

Authors:  Daniel R Matute; Lina M Quesada-Ocampo; Jason T Rauscher; Juan G McEwen
Journal:  PLoS Negl Trop Dis       Date:  2008-09-17
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.