| Literature DB >> 8989203 |
K R Porter1, J R Mascola, H Hupudio, D Ewing, T C VanCott, R L Anthony, A L Corwin, S Widodo, S Ertono, F E McCutchan, D S Burke, C G Hayes, F S Wignall, R R Graham.
Abstract
To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.Entities:
Keywords: Antigens; Asia; Biology; Developing Countries; Diseases; Examinations And Diagnoses; Genetics; Hiv; Hiv Infections; Hiv Serodiagnosis; Immunity; Immunologic Factors; Indonesia; Laboratory Examinations And Diagnoses; Laboratory Procedures; Physiology; Research Methodology; Research Report; Sampling Studies; Southeastern Asia; Studies; Viral Diseases
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Year: 1997 PMID： 8989203 DOI： 10.1097/00042560-199701010-00001
Source DB: PubMed Journal: J Acquir Immune Defic Syndr Hum Retrovirol ISSN： 1077-9450