Nonrecombinant and recombinant avirulent Salmonella vaccines for poultry.

We have constructed and evaluated a live avirulent Salmonella typhimurium vaccine strain, attenuated by deletion (delta) mutations in genes for adenylate cyclase (cya) and the cAMP receptor protein (crp). Immunization of chicks can preclude Salmonella colonization and invasion of challenged vaccinated chickens when compared with the non vaccinated control. Immunization induces significant cross-protective immunity against various Salmonella serotypes and protects laying hens from transmission of Salmonella in or on eggs following challenge with S. enteritidis or S. typhimurium. Immunization of chicks destined to be breeders and then with a booster immunization at 16-18 weeks of age leads to maternal transfer of immunity to chicks which then can be immunized either orally or by coarse spray to display an enhanced immunity to prevent infection of visceral organs by and shedding of Salmonella. The attenuated S. typhimurium vaccine can be genetically manipulated to express foreign antigens specified by cloned genes from other pathogens. Immunization with such recombinant vaccines not only induces immunity to Salmonella but to infection by the pathogen that supplied the genes specifying the protective antigens expressed by the recombinant vaccine.