Literature DB >> 8987894

Cyanobacteria carrying an smt-lux transcriptional fusion as biosensors for the detection of heavy metal cations.

J L Erbe1, A C Adams, K B Taylor, L M Hall.   

Abstract

The metal-responsive smt operator/promoter region of Synechococcus PCC7942 was fused to the luxCDABE genes of Vibrio fischeri. Plasmid DNA (pJLE23) carrying this fusion conferred metal ion-inducible luminescence to transformed cyanobacteria. Synechococcus PCC7942 (pJLE23) was sensitive to ZnCl2 concentrations within a range of 0.5-4 microM as demonstrated by induction of luminescence. Trace levels of CuSO24 and CdCl2 were also detected.

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Year:  1996        PMID： 8987894     DOI： 10.1007/bf01570047

Source DB:  PubMed          Journal:  J Ind Microbiol        ISSN： 0169-4146


  11 in total

1.  Construction and evaluation of a self-luminescent biosensor.

Authors:  L Geiselhart; M Osgood; D S Holmes
Journal:  Ann N Y Acad Sci       Date:  1991-12-27       Impact factor: 5.691

2.  Host-vector systems for gene cloning in cyanobacteria.

Authors:  C J Kuhlemeier; G A van Arkel
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

3.  Metalloregulation of the cyanobacterial smt locus: identification of SmtB binding sites and direct interaction with metals.

Authors:  J L Erbe; K B Taylor; L M Hall
Journal:  Nucleic Acids Res       Date:  1995-07-11       Impact factor: 16.971

Review 4.  Cyanobacterial metallothioneins: biochemistry and molecular genetics.

Authors:  J S Turner; N J Robinson
Journal:  J Ind Microbiol       Date:  1995-02

5.  SmtB is a metal-dependent repressor of the cyanobacterial metallothionein gene smtA: identification of a Zn inhibited DNA-protein complex.

Authors:  A P Morby; J S Turner; J W Huckle; N J Robinson
Journal:  Nucleic Acids Res       Date:  1993-02-25       Impact factor: 16.971

6.  Bioluminescent sensors for detection of bioavailable Hg(II) in the environment.

Authors:  O Selifonova; R Burlage; T Barkay
Journal:  Appl Environ Microbiol       Date:  1993-09       Impact factor: 4.792

7.  Isolation of a prokaryotic metallothionein locus and analysis of transcriptional control by trace metal ions.

Authors:  J W Huckle; A P Morby; J S Turner; N J Robinson
Journal:  Mol Microbiol       Date:  1993-01       Impact factor: 3.501

8.  Transcription of the Escherichia coli fliC gene is regulated by metal ions.

Authors:  A Guzzo; C Diorio; M S DuBow
Journal:  Appl Environ Microbiol       Date:  1991-08       Impact factor: 4.792

9.  luxAB gene fusions with the arsenic and cadmium resistance operons of Staphylococcus aureus plasmid pI258.

Authors:  P Corbisier; G Ji; G Nuyts; M Mergeay; S Silver
Journal:  FEMS Microbiol Lett       Date:  1993-06-15       Impact factor: 2.742

Review 10.  Plasmids for heavy metal resistance in Alcaligenes eutrophus CH34: mechanisms and applications.

Authors:  J M Collard; P Corbisier; L Diels; Q Dong; C Jeanthon; M Mergeay; S Taghavi; D van der Lelie; A Wilmotte; S Wuertz
Journal:  FEMS Microbiol Rev       Date:  1994-08       Impact factor: 16.408
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  7 in total

1.  Zn2+-Inducible Expression Platform for Synechococcus sp. Strain PCC 7002 Based on the smtA Promoter/Operator and smtB Repressor.

Authors:  Adam A Pérez; John P Gajewski; Bryan H Ferlez; Marcus Ludwig; Carol S Baker; John H Golbeck; Donald A Bryant
Journal:  Appl Environ Microbiol       Date:  2017-01-17       Impact factor: 4.792

Review 2.  The evolution of the bacterial luciferase gene cassette (lux) as a real-time bioreporter.

Authors:  Dan Close; Tingting Xu; Abby Smartt; Alexandra Rogers; Robert Crossley; Sarah Price; Steven Ripp; Gary Sayler
Journal:  Sensors (Basel)       Date:  2012-01-11       Impact factor: 3.576

3.  Construction of a self-luminescent cyanobacterial bioreporter that detects a broad range of bioavailable heavy metals in aquatic environments.

Authors:  Keila Martín-Betancor; Ismael Rodea-Palomares; M A Muñoz-Martín; Francisco Leganés; Francisca Fernández-Piñas
Journal:  Front Microbiol       Date:  2015-03-09       Impact factor: 5.640

4.  Determination of zinc, cadmium and lead bioavailability in contaminated soils at the single-cell level by a combination of whole-cell biosensors and flow cytometry.

Authors:  Quentin Hurdebise; Cédric Tarayre; Christophe Fischer; Gilles Colinet; Serge Hiligsmann; Frank Delvigne
Journal:  Sensors (Basel)       Date:  2015-04-16       Impact factor: 3.576

Review 5.  Synthetic biology of cyanobacteria: unique challenges and opportunities.

Authors:  Bertram M Berla; Rajib Saha; Cheryl M Immethun; Costas D Maranas; Tae Seok Moon; Himadri B Pakrasi
Journal:  Front Microbiol       Date:  2013-08-27       Impact factor: 5.640

6.  AraBAD Based Toolkit for Gene Expression and Metabolic Robustness Improvement in Synechococcus elongatus.

Authors:  Yi-Qi Cao; Qian Li; Peng-Fei Xia; Liu-Jing Wei; Ning Guo; Jian-Wei Li; Shu-Guang Wang
Journal:  Sci Rep       Date:  2017-12-22       Impact factor: 4.379

7.  Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 7942.

Authors:  Yi Ern Cheah; Yao Xu; Sarah A Sacco; Piyoosh K Babele; Amy O Zheng; Carl Hirschie Johnson; Jamey D Young
Journal:  Metab Eng       Date:  2020-03-25       Impact factor: 9.783
  7 in total

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