OBJECTIVE: To investigate the use of a simplified short-term coculture system with luteinized granulosa cells (GCs) in patients with failed IVF-ET. DESIGN: Controlled clinical study. SETTING: IVF unit, Department of Obstetrics and Gynecology, Carmel Medical Center. PATIENT(S): Patients with poor embryo quality in their previous IVF-ET cycles. INTERVENTION(S): Embryos from 40 patients, in which > 50% of the embryos were classified as poor quality in their previous IVF attempts, were grown on autologue GC culture system for a short period (24-48 hours) before being replaced in the uterine cavity. MAIN OUTCOME MEASURE(S): Embryo quality. RESULT(S): Significant decrease in poor quality embryos and increase in the proportion of good quality embryos were observed using a coculture system with autologue human GCs. Pregnancy rates in this groups of patients reached our standard IVF results during the same period. CONCLUSION(S): This study describes a simplified short-term coculture system with human autologue GCs. Poor quality embryos may be rescued to cleave regularly.
OBJECTIVE: To investigate the use of a simplified short-term coculture system with luteinized granulosa cells (GCs) in patients with failed IVF-ET. DESIGN: Controlled clinical study. SETTING: IVF unit, Department of Obstetrics and Gynecology, Carmel Medical Center. PATIENT(S): Patients with poor embryo quality in their previous IVF-ET cycles. INTERVENTION(S): Embryos from 40 patients, in which > 50% of the embryos were classified as poor quality in their previous IVF attempts, were grown on autologue GC culture system for a short period (24-48 hours) before being replaced in the uterine cavity. MAIN OUTCOME MEASURE(S): Embryo quality. RESULT(S): Significant decrease in poor quality embryos and increase in the proportion of good quality embryos were observed using a coculture system with autologue human GCs. Pregnancy rates in this groups of patients reached our standard IVF results during the same period. CONCLUSION(S): This study describes a simplified short-term coculture system with human autologue GCs. Poor quality embryos may be rescued to cleave regularly.
Authors: D T Carrell; C M Peterson; K P Jones; H H Hatasaka; L C Udoff; C E Cornwell; C Thorp; P Kuneck; L Erickson; B Campbell Journal: J Assist Reprod Genet Date: 1999-08 Impact factor: 3.412
Authors: R Fabbri; E Porcu; T Marsella; M R Primavera; S Cecconi; S A Nottola; P M Motta; S Venturoli; C Flamigni Journal: J Assist Reprod Genet Date: 2000-01 Impact factor: 3.412