Literature DB >> 8985979

Purification of transforming growth factor beta 1 from human platelets.

O Werz1, M Brungs, D Steinhilber.   

Abstract

Transforming growth factor-beta (TGF beta) is a growth and differentiation factor which can be released from many cell types. In previous studies, platelets were identified as a rich source of TGF beta. Here we present a rapid and convenient method for TGF beta purification from human platelets which includes acid-ethanol extraction and gelfiltration, cation exchange and reversed phase chromatography. All purification steps are performed under acidic conditions to prevent adsorption of TGF beta to the vial walls. In addition, volatile solvents and buffers were used which allowed easy removal of solvent and salt by lyophilization. Using this method pure TGF beta can be easily obtained in high yield (370 micrograms) from 20 units of platelet concentrate.

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Year:  1996        PMID： 8985979

Source DB:  PubMed          Journal:  Pharmazie        ISSN： 0031-7144            Impact factor:   1.267


  8 in total

1.  Molecular pharmacological profile of a novel thiazolinone-based direct and selective 5-lipoxygenase inhibitor.

Authors:  B Hofmann; C B Rödl; A S Kahnt; T J Maier; A A Michel; M Hoffmann; O Rau; K Awwad; M Pellowska; M Wurglics; M Wacker; A Zivković; I Fleming; M Schubert-Zsilavecz; H Stark; G Schneider; D Steinhilber
Journal:  Br J Pharmacol       Date:  2012-04       Impact factor: 8.739

2.  5-Lipoxygenase inhibitors induce potent anti-proliferative and cytotoxic effects in human tumour cells independently of suppression of 5-lipoxygenase activity.

Authors:  A S Fischer; J Metzner; S D Steinbrink; S Ulrich; C Angioni; G Geisslinger; D Steinhilber; T J Maier
Journal:  Br J Pharmacol       Date:  2010-10       Impact factor: 8.739

3.  Evidence that furin is an authentic transforming growth factor-beta1-converting enzyme.

Authors:  C M Dubois; F Blanchette; M H Laprise; R Leduc; F Grondin; N G Seidah
Journal:  Am J Pathol       Date:  2001-01       Impact factor: 4.307

4.  Glutathione peroxidase-1 but not -4 is involved in the regulation of cellular 5-lipoxygenase activity in monocytic cells.

Authors:  D Straif; O Werz; R Kellner; U Bahr; D Steinhilber
Journal:  Biochem J       Date:  2000-07-15       Impact factor: 3.857

5.  Coupling of boswellic acid-induced Ca2+ mobilisation and MAPK activation to lipid metabolism and peroxide formation in human leucocytes.

Authors:  Anja Altmann; Daniel Poeckel; Lutz Fischer; Manfred Schubert-Zsilavecz; Dieter Steinhilber; Oliver Werz
Journal:  Br J Pharmacol       Date:  2003-12-22       Impact factor: 8.739

6.  Inhibition of class I HDACs abrogates the dominant effect of MLL-AF4 by activation of wild-type MLL.

Authors:  K Ahmad; C Katryniok; B Scholz; J Merkens; D Löscher; R Marschalek; D Steinhilber
Journal:  Oncogenesis       Date:  2014-11-17       Impact factor: 7.485

7.  CD69 is a TGF-β/1α,25-dihydroxyvitamin D3 target gene in monocytes.

Authors:  Thea K Wöbke; Andreas von Knethen; Dieter Steinhilber; Bernd L Sorg
Journal:  PLoS One       Date:  2013-05-16       Impact factor: 3.240

8.  AF4 and AF4-MLL mediate transcriptional elongation of 5-lipoxygenase mRNA by 1, 25-dihydroxyvitamin D3.

Authors:  Khalil Ahmad; Bastian Scholz; Ricardo Capelo; Ilona Schweighöfer; Astrid Stefanie Kahnt; Rolf Marschalek; Dieter Steinhilber
Journal:  Oncotarget       Date:  2015-09-22
  8 in total

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