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Literature DB >> 8983026

Methylation status of Sillago japonica satellite DNA examined by bisulfite modification.

Abstract

A member of Sillago japonica satellite DNA contained internal subrepeats in its 174 bp unit. S. Japonica genomic DNA isolated from liver tissue was subjected to bisulfite modification, and the DNA sequences of about 40 bp flanked by both subrepeats were amplified by polymerase chain reaction (PCR). This protocol, combination of bisulfite reaction and PCR, converts cytosines in the genomic DNA to thymines in the amplified DNA, whereas 5-methylcytosines in the genomic DNA remain as cytosines. Sequence analysis of the amplified DNA fragments revealed that most of the cytosine residues at CpG were methylated in this region.

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Year: 1996 PMID： 8983026 DOI： 10.1007/bf00424438

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

18 in total

1. CpNpG methylation in mammalian cells.

Authors: S J Clark; J Harrison; M Frommer
Journal: Nat Genet Date: 1995-05 Impact factor: 38.330

2. Effect of regional DNA methylation on gene expression.

Authors: I Keshet; J Yisraeli; H Cedar
Journal: Proc Natl Acad Sci U S A Date: 1985-05 Impact factor: 11.205

3. Two highly reiterated nucleotide sequences in the low C-value genome of Panagrellus redivivus.

Authors: Y de Chastonay; F Müller; H Tobler
Journal: Gene Date: 1990-09-14 Impact factor: 3.688

4. An AT-rich satellite DNA sequence, E180, in alfalfa (Medicago sativa).

Authors: X Xia; L Erickson
Journal: Genome Date: 1993-06 Impact factor: 2.166

5. Abnormal methylation pattern in constitutive and facultative (X inactive chromosome) heterochromatin of ICF patients.

Authors: P Miniou; M Jeanpierre; V Blanquet; V Sibella; D Bonneau; C Herbelin; A Fischer; A Niveleau; E Viegas-Péquignot
Journal: Hum Mol Genet Date: 1994-12 Impact factor: 6.150

Review 6. DNA methylation and cancer.

Authors: P W Laird; R Jaenisch
Journal: Hum Mol Genet Date: 1994 Impact factor: 6.150

Methylation status of Sillago japonica satellite DNA examined by bisulfite modification.

1. CpNpG methylation in mammalian cells.

2. Effect of regional DNA methylation on gene expression.

3. Two highly reiterated nucleotide sequences in the low C-value genome of Panagrellus redivivus.

4. An AT-rich satellite DNA sequence, E180, in alfalfa (Medicago sativa).

5. Abnormal methylation pattern in constitutive and facultative (X inactive chromosome) heterochromatin of ICF patients.

Review 6. DNA methylation and cancer.

7. Active maize genes are unmodified and flanked by diverse classes of modified, highly repetitive DNA.

8. High sensitivity mapping of methylated cytosines.

9. Molecular and physical organization of highly repetitive, undermethylated DNA from Pennisetum glaucum.

1. Novel methylation at GpC dinucleotide in the fish Sparus aurata genome.